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PMID: 11196711 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SPLASH (PLA2IID), a novel member of phospholipase A2 family, is associated with lymphotoxin deficiency.

Genes and immunity ·Vol. 1 ·No. 3 ·2000-02-00 ·Pages 191-9

Shakhov AN, Rubtsov AV, Lyakhov IG, Tumanov AV, Nedospasov SA

Abstract

Lymphotoxin (LT) deficient mice have profound defects in the splenic microarchitecture associated with defective expression on certain gene products, including chemokines. By using subtraction cloning of splenic cDNA from wild-type and LT alpha or TNF/LT alpha double deficient mice we isolated a novel murine gene encoding a secretory type phospholipase A2, called SPLASH. The two major alternative transcripts of SPLASH gene are predominantly expressed in lymphoid tissues, such as spleen and lymph nodes. SPLASH maps to the distal part of chromosome 4, to which several cancer-related loci have been also mapped.

MeSH Terms

Alternative Splicing Amino Acid Sequence Animals Base Sequence Chromosome Mapping Chromosomes, Human, Pair 1/genetics Cloning, Molecular DNA Primers/genetics DNA, Complementary/genetics Gene Expression Group II Phospholipases A2 Humans Lymphoid Tissue/enzymology,immunology Lymphotoxin-alpha/metabolism Mice Mice, Inbred C57BL Mice, Knockout Molecular Sequence Data Phospholipases A/genetics Phospholipases A2 Sequence Homology, Amino Acid Species Specificity

Chemicals

DNA Primers DNA, Complementary Lymphotoxin-alpha Phospholipases A Group II Phospholipases A2 PLA2G2D protein, human Phospholipases A2 Pla2g2d protein, mouse

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Shakhov A N

Intramural Research Support Program, SAIC Frederick and Laboratory of Molecular Immunoregulation, Division of Basic Sciences, NCI-FCRDC, Frederick, MD 21702, USA. shakhova@mail.ncifcrf.gov

Rubtsov A V

Lyakhov I G

Tumanov A V

Nedospasov S A

Article Info

Journal

Genes and immunity

Abbr.

Genes Immun

ISSN

1466-4879

DOI

10.1038/sj.gene.6363659

shakhova@mail.ncifcrf.gov

Published

2000-02-00

Pages

191-9

Language

English

Region

England

NLM ID

100953417

Subset

Grants

NCI NIH HHS · N01-CO-56000 · United States

External Links

PubMed source DOI: 10.1038/sj.gene.6363659 View journal details

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SPLASH (PLA2IID), a novel member of phospholipase A2 family, is associated with lymphotoxin deficiency.

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