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PMID: 1117486 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of the inhomogeneous DNA in virions of bacteriophage Mu by DNA reannealing kinetics.

Journal of virology ·Vol. 15 ·No. 4 ·1975-04-00 ·Pages 739-43

Daniell E, Kohne DE, Abelson J

Abstract

The DNA of bacteriophage Mu has been studied to characterize a region of inhomogeneous sequence that occurs at one end of the molecule. The kinetics of reassocation of tracer amounts of labeled host DNA in the presence of Mu DNA show that Mu DNA contains a complete selection of host sequences. These host sequences are shown to be covalently attached to phage-specific sequences and are present at a concentration that accounts for the inhomogeneity observed in the electron microscope. The significance and possible function of the host DNA attachment is discussed.

MeSH Terms

Base Sequence Coliphages/analysis DNA, Viral/analysis Kinetics Nucleic Acid Renaturation Phosphorus Radioisotopes

Chemicals

DNA, Viral Phosphorus Radioisotopes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Daniell E

Kohne D E

Abelson J

References (18)

18 references, click to expand

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Methods Enzymol. 1974;29:363-418 PMID: 4850571
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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.15.4.739-743.1975

Published

1975-04-00

Pages

739-43

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC354517

Subset

External Links

PubMed source DOI: 10.1128/JVI.15.4.739-743.1975 PMC Full Text View journal details

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Characterization of the inhomogeneous DNA in virions of bacteriophage Mu by DNA reannealing kinetics.

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