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PMID: 11163772 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of two proteins, S14 and UIP1, that interact with UCH37.

FEBS letters ·Vol. 488 ·No. 3 ·2001-01-19 ·Pages 201-5

Li T, Duan W, Yang H, Lee MK, Bte Mustafa F, Lee BH, Teo TS

Abstract

By the use of the yeast two-hybrid screen we have identified two proteins that interacted with UCH37: S14, which is a subunit of PA700 and a novel protein, UIP1 (UCH37 interacting protein 1). The interaction of UCH37 with S14 or UIP1 was confirmed by in vitro binding assay and in vivo co-immunoprecipitation analysis. The C-terminal extension of UCH37 is essential for interaction with S14 or UIP1 as shown by the yeast two-hybrid assay and the in vitro binding assay. Furthermore, UIP1 blocked the interaction between UCH37 and S14 in vitro.

MeSH Terms

Amino Acid Sequence Binding Sites Binding, Competitive Blotting, Western Carboxypeptidases Carrier Proteins/chemistry,genetics,metabolism Gene Expression Profiling Humans Molecular Sequence Data Precipitin Tests Proteasome Endopeptidase Complex Protein Binding Protein Subunits Proteins/chemistry,genetics,metabolism RNA, Messenger/analysis,genetics Sequence Alignment Thiolester Hydrolases/chemistry,genetics,metabolism Two-Hybrid System Techniques Ubiquitin Thiolesterase

Chemicals

Carrier Proteins PA700 proteasome activator Protein Subunits Proteins RNA, Messenger Thiolester Hydrolases Carboxypeptidases UCHL5 protein, human Ubiquitin Thiolesterase Proteasome Endopeptidase Complex

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Li T

Department of Biochemistry, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore, Singapore.

Duan W

Yang H

Lee M K

Bte Mustafa F

Lee B H

Teo T S

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/s0014-5793(00)02436-4

Published

2001-01-19

Pages

201-5

Language

English

Region

England

NLM ID

0155157

Subset

Databases

GENBANK

AF267739, U82695

External Links

PubMed source DOI: 10.1016/s0014-5793(00)02436-4 View journal details

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Identification of two proteins, S14 and UIP1, that interact with UCH37.

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