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PMID: 11157271 Published · ppublish English Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR.

Applied and environmental microbiology ·Vol. 67 ·No. 2 ·2001-02-00 ·Pages 972-6

Hermansson A, Lindgren PE

Abstract

Real-time PCR was used to quantify populations of ammonia-oxidizing bacteria representing the beta subdivision of the class Proteobacteria in samples of arable soil, both nitrogen fertilized and unfertilized, from Mellby, Sweden. Primers and probes targeting a 16S ribosomal DNA region of the ammonia-oxidizing bacteria were designed and used. In the fertilized soil there were approximately 6.2 x 10(7) ammonia-oxidizing bacteria per g of soil, three times more than the number of bacteria in the unfertilized soil. The lytic efficiency of bead beating in these soils was investigated by using populations of free or loosely attached bacteria, bacteria tightly bound to particles, and bacteria in nonfractionated samples. The shapes of the curves generated in these tests showed that the concentration of template DNA released at various times remained constant after 10 to 100 s of bead beating.

MeSH Terms
Ammonia/metabolism DNA, Ribosomal/analysis Oxidation-Reduction Polymerase Chain Reaction/methods Proteobacteria/isolation & purification,metabolism RNA, Ribosomal, 16S/genetics Soil Microbiology
Chemicals
DNA, Ribosomal RNA, Ribosomal, 16S Ammonia
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hermansson A
Department of Biology, Linköping University, S-58183 Linköping, Sweden.
Lindgren P E
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.67.2.972-976.2001
Published
2001-02-00
Pages
972-6
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC92675
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.67.2.972-976.2001 PMC Full Text View journal details
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