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PMID: 11155981 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis.

International journal of systematic and evolutionary microbiology ·Vol. 50 Pt 6 ·2000-11-00 ·Pages 2069-2073

Figueras MJ, Soler L, Chacón MR, Guarro J, Martínez-Murcia AJ

Abstract

A previously described molecular method, based on 16S rDNA RFLP analysis, for the identification of Aeromonas spp. was unable to separate the species Aeromonas salmonicida, Aeromonas bestiarum and the recently described Aeromonas popoffii. In this study, the method has been extended with endonucleases AIwNI and PstI for the identification of these species. A molecular frame for the identification of all known Aeromonas spp. is presented.

MeSH Terms

Aeromonas/classification,genetics Animals DNA, Ribosomal/analysis Deoxyribonucleases, Type II Site-Specific/metabolism Humans Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA

Chemicals

DNA, Ribosomal RNA, Ribosomal, 16S endodeoxyribonuclease AlwNI CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Figueras M J

Soler L

Chacón M R

Guarro J

Martínez-Murcia A J

Article Info

Journal

International journal of systematic and evolutionary microbiology

Abbr.

Int J Syst Evol Microbiol

ISSN

1466-5026

DOI

10.1099/00207713-50-6-2069

Published

2000-11-00

Pages

2069-2073

Language

English

Region

England

NLM ID

100899600

Subset

External Links

PubMed source DOI: 10.1099/00207713-50-6-2069 View journal details

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Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis.

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