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PMID: 11139080 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui.

Extremophiles : life under extreme conditions ·Vol. 4 ·No. 6 ·2000-12-00 ·Pages 373-6

Amann G, Stetter KO, Llobet-Brossa E, Amann R, Antón J

Abstract

A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.

MeSH Terms

Base Sequence DNA Primers Haloarcula marismortui/genetics In Situ Hybridization, Fluorescence Phylogeny RNA, Ribosomal, 16S/genetics

Chemicals

DNA Primers RNA, Ribosomal, 16S

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Amann G

Lehrstuhl für Mikrobiologie, Universität Regensburg, Germany.

Stetter K O

Llobet-Brossa E

Amann R

Antón J

Article Info

Journal

Extremophiles : life under extreme conditions

Abbr.

Extremophiles

ISSN

1431-0651

DOI

10.1007/s007920070007

Published

2000-12-00

Pages

373-6

Language

English

Region

Germany

NLM ID

9706854

Subset

External Links

PubMed source DOI: 10.1007/s007920070007 View journal details

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Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui.

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