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PMID: 1112775 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Citrate uptake in membrane vesicles of Klebsiella aerogenes.

Journal of bacteriology ·Vol. 121 ·No. 2 ·1975-02-00 ·Pages 682-7

Johnson CL, Cha YA, Stern JR

Abstract

In whole cells of Klebsiella aerogenes grown anaerobically on citrate as sole carbon source, citrate uptake is followed by rapid catabolism of the substrate via the inducible citrate fermentation pathway. Membrane vesicles prepared from such cells take up citrate but do not catabolize it. Vesicles process d-lactate dehydrogenase and the Na+-requiring oxalacetate decarboxylase. Citrate is taken up in the presence of Na+, and other monovalent cations, such as NH4+, Rb+, Cs+, or K+, do not substitute for Na+. Li+ appears to act synergistically with Na+. Citrate uptake is inhibited by N-2, cyanide, azide, sulfhydryl reagents, dinitrophenol, fluorcitrate, and hydroxycitrate.

MeSH Terms

Anaerobiosis Azides/pharmacology Carbon Radioisotopes Carboxy-Lyases/metabolism Cell Fractionation Cell Membrane/enzymology,metabolism Cell-Free System Citrates/metabolism Cyanides/pharmacology Dinitrophenols/pharmacology Electron Transport Klebsiella/enzymology,metabolism L-Lactate Dehydrogenase/metabolism Lactates/metabolism Malate Dehydrogenase/metabolism Oxo-Acid-Lyases/metabolism Proline/metabolism Spectrophotometry Stereoisomerism Sulfhydryl Reagents/pharmacology

Chemicals

Azides Carbon Radioisotopes Citrates Cyanides Dinitrophenols Lactates Sulfhydryl Reagents Proline L-Lactate Dehydrogenase Malate Dehydrogenase Carboxy-Lyases Oxo-Acid-Lyases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Johnson C L

Cha Y A

Stern J R

References (14)

14 references, click to expand

Dissimilation of citric acid by bacterial extracts.
Nature. 1953 Aug 15;172(4372):345-6 PMID: 13087223
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Citrate-Mg2+ transport in Bacillus subtilis. Studies with 2-fluoro-L-erythro-citrate as a substrate.
J Biol Chem. 1974 Apr 10;249(7):2037-42 PMID: 4206548
Anaerobic transport in Escherichia coli membrane vesicles.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3376-81 PMID: 4587250
Mechanisms of active transport in isolated bacterial membrane vesicles. 8. The transport of amino acids by membranes prepared from Escherichia coli.
J Biol Chem. 1972 Dec 25;247(24):7844-57 PMID: 4344983
Coupled transport of citrate and magnesium in Bacillus subtilis.
J Biol Chem. 1973 Feb 10;248(3):807-14 PMID: 4630854
Requirement for sodium in the anaerobic growth of Aerobacter aerogenes on citrate.
J Bacteriol. 1969 May;98(2):388-93 PMID: 5784198
A potassium-dependent citric acid transport system in Aerobacter aerogenes.
Biochem Biophys Res Commun. 1972 Mar 10;46(5):1944-50 PMID: 5015237
Transport of citric acid by Aerobacter aerogenes.
Arch Biochem Biophys. 1972 Mar;149(1):209-21 PMID: 5017252
Inducible transport of citrate in a Gram-positive bacterium, Bacillus subtilis.
J Biol Chem. 1971 Feb 25;246(4):1032-40 PMID: 4993437
Oxalacetate decarboxylase of Aerobacter aerogenes. I. Inhibition by avidin and requirement for sodium ion.
Biochemistry. 1967 Nov;6(11):3545-51 PMID: 4965256
Studies of citrate transport in Aerobacter aerogenes: binding of citrate by a membrane bound oxalacetate decarboxylase.
Biochem Biophys Res Commun. 1971 Oct 15;45(2):402-8 PMID: 5160725
The transport of citrate and other tricarboxylic acids in two species of Pseudomonas.
Biochem J. 1971 Jul;123(4):571-7 PMID: 5126909
Effect of sodium on the transport and utilization of citric acid by Aerobacter (Enterobacter) aerogenes.
J Bacteriol. 1974 Oct;120(1):121-4 PMID: 4418533

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.121.2.682-687.1975

Published

1975-02-00

Pages

682-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC245982

Subset

External Links

PubMed source DOI: 10.1128/jb.121.2.682-687.1975 PMC Full Text View journal details

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Citrate uptake in membrane vesicles of Klebsiella aerogenes.

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