Abstract
Endotoxic lipopolysachharide (LPS) was obtained from phenol-water extraction of cell walls prepared from mass-cultivated Fusobacterium necrophorum. The LPS was relatively free of nucleic acids and low in protein, and constituted about 4% of the cell walls. Upon acid hydrolysis, some of the components detected were hexosamines (7.0%), neutral and reducing sugars (50.5%), heptose (6.4%), 2-keto-3-deoxyoctonate (0.8%), lipid A (21.0%), and phosphorus (1.7%). Under electron microscopy the LPS appeared mainly as ribbon-like trilaminar structures, and upon chemical treatment it displayed a behavior resembling that reported in certain enterobacterial LPS. The LPS was lethal to mice, 11-day-old chicken embryos, and rabbits. Endotoxicity in mice was enhanced at least 1,380-fold by the addition of 12.5 mug of actinomycin D. Induced tolerance to lethal effect of the endotoxin and rapidly acquired resistance to infection by F. necrophrum viable cells were also demonstrated in mice. The endotoxin produced both localized and generalized Shwartzman reactions as well as biphasic pyrogenic responses in rabbits. These results firmly establish the presence of a classical endotoxin in F. necrophorum, thus providing strong support to our recent suggestion that cell wall-associated components may contribute significantly to the pathogenicity of F. necrophorum.
MeSH Terms
Animals
Bacterial Proteins/analysis
Cell Wall/immunology
Chick Embryo
Dactinomycin/pharmacology
Endotoxins/analysis,isolation & purification,toxicity
Female
Fusobacterium/immunology
Heptoses/analysis
Hexosamines/analysis
Immune Tolerance
Immunity
Immunization
Lethal Dose 50
Lipopolysaccharides/analysis
Mice
Microscopy, Electron
Phenols
Phosphorus/analysis
Polysaccharides, Bacterial/analysis
Pyrogens
Rabbits
Shwartzman Phenomenon
Chemicals
Bacterial Proteins
Endotoxins
Heptoses
Hexosamines
Lipopolysaccharides
Phenols
Polysaccharides, Bacterial
Pyrogens
Dactinomycin
Phosphorus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Garcia M M
Charlton K M
McKay K A
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16 references, click to expand
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