Home LiteratureArticle Details

PMID: 11063690 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

New insights on homology-dependent silencing of I factor activity by transgenes containing ORF1 in Drosophila melanogaster.

Genetics ·Vol. 156 ·No. 3 ·2000-11-00 ·Pages 1147-55

Malinsky S, Bucheton A, Busseau I

Abstract

I factors in Drosophila melanogaster are non-LTR retrotransposons that transpose at very high frequencies in the germ line of females resulting from crosses between reactive females (devoid of active I factors) and inducer males (containing active I factors). Constructs containing I factor ORF1 under the control of the hsp70 promoter repress I factor activity. This repressor effect is maternally transmitted and increases with the transgene copy number. It is irrespective of either frame integrity or transcriptional orientation of ORF1, suggesting the involvement of a homology-dependent trans-silencing mechanism. A promoterless transgene displays no repression. The effect of constructs in which ORF1 is controlled by the hsp70 promoter does not depend upon heat-shock treatments. No effect of ORF1 is detected when it is controlled by the I factor promoter. We discuss the relevance of the described regulation to the repression of I factors in I strains.

MeSH Terms

Animals Animals, Genetically Modified Crosses, Genetic Drosophila melanogaster/genetics Female Fertility/genetics Gene Silencing Male Promoter Regions, Genetic Proteins/genetics Recombination, Genetic Regulatory Sequences, Nucleic Acid Retroelements/genetics Transcription, Genetic

Chemicals

Proteins Retroelements

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Malinsky S

Institut de Génétique Humaine, CNRS, 34396 Montpellier Cedex 05, France.

Bucheton A

Busseau I

References (22)

22 references, click to expand

Targeted mRNA degradation by double-stranded RNA in vitro.
Genes Dev. 1999 Dec 15;13(24):3191-7 PMID: 10617568
Cosuppression of nonhomologous transgenes in Drosophila involves mutually related endogenous sequences.
Cell. 1999 Oct 1;99(1):35-46 PMID: 10520992
Transposable elements controlling I-R hybrid dysgenesis in D. melanogaster are similar to mammalian LINEs.
Cell. 1986 Dec 26;47(6):1007-15 PMID: 2430722
A cloned I-factor is fully functional in Drosophila melanogaster.
Mol Gen Genet. 1988 Nov;214(3):533-40 PMID: 2851098
A long interspersed repetitive element--the I factor of Drosophila teissieri--is able to transpose in different Drosophila species.
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8887-91 PMID: 2554335
Identification of a potential RNA intermediate for transposition of the LINE-like element I factor in Drosophila melanogaster.
EMBO J. 1990 Nov;9(11):3557-63 PMID: 1698618
Spatial and temporal expression of the I factor during oogenesis in Drosophila melanogaster.
Development. 1992 Jul;115(3):729-35 PMID: 1330483
Germ-line expression of the I factor, a functional LINE from the fruit fly Drosophila melanogaster, is positively regulated by reactivity, a peculiar cellular state.
Mol Gen Genet. 1993 Aug;240(2):277-85 PMID: 8394985
Retrotransposition of a marked Drosophila line-like I element in cells in culture.
Nucleic Acids Res. 1994 Apr 25;22(8):1484-8 PMID: 8190641
The full-length transcript of the I factor, a LINE element of Drosophila melanogaster, is a potential bicistronic RNA messenger.
Nucleic Acids Res. 1994 Jun 25;22(12):2370-4 PMID: 8036166
I factors in Drosophila melanogaster: transposition under control.
Genetica. 1994;93(1-3):101-16 PMID: 7813907
Defective I elements introduced into Drosophila as transgenes can regulate reactivity and prevent I-R hybrid dysgenesis.
Mol Gen Genet. 1995 Aug 30;248(4):381-90 PMID: 7565601
A genetically tagged, defective I element can be complemented by actively transposing I factors in the germline of I-R dysgenic females in Drosophila melanogaster.
Mol Gen Genet. 1995 Aug 30;248(4):434-8 PMID: 7565607
Cosuppression in Drosophila: gene silencing of Alcohol dehydrogenase by white-Adh transgenes is Polycomb dependent.
Cell. 1997 Aug 8;90(3):479-90 PMID: 9267028
A genetically marked I element in Drosophila melanogaster can be mobilized when ORF2 is provided in trans.
Genetics. 1998 Jan;148(1):267-75 PMID: 9475738
Copy number control of a transposable element, the I factor, a LINE-like element in Drosophila.
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11781-5 PMID: 9751742
The rde-1 gene, RNA interference, and transposon silencing in C. elegans.
Cell. 1999 Oct 15;99(2):123-32 PMID: 10535731
Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD.
Cell. 1999 Oct 15;99(2):133-41 PMID: 10535732
Cosuppression of I transposon activity in Drosophila by I-containing sense and antisense transgenes.
Genetics. 1999 Dec;153(4):1767-74 PMID: 10581283
Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway.
Cell. 1998 Dec 23;95(7):1017-26 PMID: 9875855
Taming of transposable elements by homology-dependent gene silencing.
Nat Genet. 1999 Feb;21(2):209-12 PMID: 9988275
The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor.
Cell. 1984 Aug;38(1):153-63 PMID: 6088060

Article Info

Journal

Genetics

Abbr.

Genetics

ISSN

0016-6731

DOI

10.1093/genetics/156.3.1147

Published

2000-11-00

Pages

1147-55

Language

English

Region

United States

NLM ID

0374636

PMCID

PMC1461323

Subset

External Links

PubMed source DOI: 10.1093/genetics/156.3.1147 PMC Full Text View journal details

Analysis Services

New insights on homology-dependent silencing of I factor activity by transgenes containing ORF1 in Drosophila melanogaster.

Contact

WeChat Official Account