Abstract
Hormone induction of growth-arrested preadipocytes triggers mitotic clonal expansion followed by expression of CCAAT/enhancer-binding protein (C/EBP)alpha and differentiation into adipocytes. The order of these events is critical because C/EBPalpha is antimitotic and its expression prematurely would block the mitotic clonal expansion required for differentiation. C/EBPbeta, a transcriptional activator of the C/EBPalpha gene, is expressed early in the differentiation program, but lacks DNA-binding activity and fails to localize to centromeres until preadipocytes traverse the G(1)-S checkpoint of mitotic clonal expansion. Evidence is presented that dominant-negative CHOP-10 expressed by growth-arrested preadipocytes transiently sequesters C/EBPbeta by heterodimerization. As preadipocytes reach S phase, CHOP-10 is down-regulated, apparently releasing C/EBPbeta from inhibitory constraint and allowing transactivation of the C/EBPalpha gene. In support of these findings, up-regulation of CHOP-10 with the protease inhibitor N-acetyl-Leu-Leu-norleucinal prevents activation of C/EBPbeta, expression of C/EBPalpha, and adipogenesis.
MeSH Terms
3T3 Cells
Adipocytes/cytology,metabolism
Animals
CCAAT-Enhancer-Binding Protein-alpha/genetics,metabolism
CCAAT-Enhancer-Binding Protein-beta/metabolism
CCAAT-Enhancer-Binding Proteins/genetics,metabolism,physiology
Cell Differentiation
Centromere/metabolism
DNA/metabolism
Down-Regulation
Mice
Phenotype
Trans-Activators/metabolism
Transcription Factor CHOP
Transcription Factors/genetics,metabolism,physiology
Up-Regulation
Chemicals
CCAAT-Enhancer-Binding Protein-alpha
CCAAT-Enhancer-Binding Protein-beta
CCAAT-Enhancer-Binding Proteins
Ddit3 protein, mouse
Trans-Activators
Transcription Factors
Transcription Factor CHOP
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tang Q Q
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21209, USA. qqtang@welchlink.welch.jhu.edu
Lane M D
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