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PMID: 11046154 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The matrix protein of vesicular stomatitis virus inhibits nucleocytoplasmic transport when it is in the nucleus and associated with nuclear pore complexes.

Molecular and cellular biology ·Vol. 20 ·No. 22 ·2000-11-00 ·Pages 8590-601

Petersen JM, Her LS, Varvel V, Lund E, Dahlberg JE

Abstract

The matrix (M) protein of vesicular stomatitis virus (VSV) is a potent inhibitor of bidirectional nuclear transport. Here we demonstrate that inhibition occurs when M protein is in the nucleus of Xenopus laevis oocytes and that M activity is readily reversed by a monoclonal antibody (alphaM). We identify a region of M protein, amino acids 51 to 59, that is required both for inhibition of transport and for efficient recognition by alphaM. When expressed in transfected HeLa cells, M protein colocalizes with nuclear pore complexes (NPCs) at the nuclear rim. Moreover, mutation of a single amino acid, methionine 51, eliminates both transport inhibition and targeting to NPCs. We propose that M protein inhibits bidirectional transport by interacting with a component of the NPC or an NPC-associated factor that participates in nucleocytoplasmic transport.

MeSH Terms
Active Transport, Cell Nucleus Amino Acid Sequence Animals Antibodies, Monoclonal/metabolism Base Sequence Cell Nucleus Structures/metabolism Cytoplasm/metabolism Female Humans Molecular Sequence Data Oocytes/metabolism Point Mutation Viral Matrix Proteins/genetics,immunology,metabolism Xenopus laevis
Chemicals
Antibodies, Monoclonal M protein, Vesicular stomatitis virus Viral Matrix Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Petersen J M
Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706-1532, USA.
Her L S
Varvel V
Lund E
Dahlberg J E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.22.8590-8601.2000
Published
2000-11-00
Pages
8590-601
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC102164
Subset
IM
Grants
NIGMS NIH HHS · R37 GM030220 · United States
NIGMS NIH HHS · T32 GM008349 · United States
NIGMS NIH HHS · GM08349-08 · United States
NIGMS NIH HHS · GM30220 · United States
External Links
PubMed source DOI: 10.1128/MCB.20.22.8590-8601.2000 PMC Full Text View journal details
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