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PMID: 11044129 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts.

Journal of virology ·Vol. 74 ·No. 22 ·2000-11-00 ·Pages 10816-8

Bresnahan WA, Hultman GE, Shenk T

Abstract

A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

MeSH Terms

Cytomegalovirus/genetics,physiology Fibroblasts/physiology,virology Humans Mutation Telomerase/genetics,metabolism Viral Proteins/genetics,metabolism Virus Replication

Chemicals

Viral Proteins cytomegalovirus phosphoprotein 71kDa Telomerase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bresnahan W A

Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA. wbresnahan@molbio.princeton.edu

Hultman G E

Shenk T

References (14)

14 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/jvi.74.22.10816-10818.2000

wbresnahan@molbio.princeton.edu

Published

2000-11-00

Pages

10816-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC110959

Subset

Grants

NCI NIH HHS · R01 CA082396 · United States

NCI NIH HHS · CA82396 · United States

External Links

PubMed source DOI: 10.1128/jvi.74.22.10816-10818.2000 PMC Full Text View journal details

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Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts.

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