Arabidopsis thaliana defense-related protein ELI3 is an aromatic alcohol:NADP+ oxidoreductase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 24 ·1996-11-26 ·Pages 14199-203

Somssich IE, Wernert P, Kiedrowski S, Hahlbrock K

Abstract

We expressed a cDNA encoding the Arabidopsis thaliana defense-related protein ELI3-2 in Escherichia coli to determine its biochemical function. Based on a protein database search, this protein was recently predicted to be a mannitol dehydrogenase [Williamson, J. D., Stoop, J. M. H., Massel, M. O., Conkling, M. A. & Pharr, D. M. (1995) Proc. Natl. Acad. Sci. USA 92, 7148-7152]. Studies on the substrate specificity now revealed that ELI3-2 is an aromatic alcohol: NADP+ oxidoreductase (benzyl alcohol dehydrogenase). The enzyme showed a strong preference for various aromatic aldehydes as opposed to the corresponding alcohols. Highest substrate affinities were observed for 2-methoxybenzaldehyde, 3-methoxybenzaldehyde, salicylaldehyde, and benzaldehyde, in this order, whereas mannitol dehydrogenase activity could not be detected. These and previous results support the notion that ELI3-2 has an important role in resistance-related aromatic acid-derived metabolism.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Somssich I E

Max Planck Institut für Züchtungsforschung, Department of Biochemistry, D-50829 Köln, Germany.

Wernert P

Kiedrowski S

Hahlbrock K

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.93.24.14199

Published

1996-11-26

Pages

14199-203

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC19517

External Links

PubMed source DOI: 10.1073/pnas.93.24.14199 PMC Full Text View journal details

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Arabidopsis thaliana defense-related protein ELI3 is an aromatic alcohol:NADP+ oxidoreductase.

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