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PMID: 11038184 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Targeted ablation of the murine involucrin gene.

The Journal of cell biology ·Vol. 151 ·No. 2 ·2000-10-16 ·Pages 381-8

Djian P, Easley K, Green H

Abstract

Involucrin is synthesized in abundance during terminal differentiation of keratinocytes. Involucrin is a substrate for transglutaminase and one of the precursors of the cross-linked envelopes present in the corneocytes of the epidermis and other stratified squamous epithelia. These envelopes make an important contribution to the physical resistance of the epidermis. We have generated mice lacking involucrin from embryonic stem cells whose involucrin gene had been ablated by homologous recombination. These mice developed normally, possessed apparently normal epidermis and hair follicles, and made cornified envelopes that could not be distinguished from those of wild-type mice. No compensatory increase of mRNA for other envelope precursors was observed.

MeSH Terms
Animals Epidermis Gene Targeting Genetic Vectors Hair Follicle Mice Mice, Mutant Strains Mutagenesis, Site-Directed Protein Precursors/genetics Reproduction Skin Physiological Phenomena/genetics
Chemicals
Protein Precursors involucrin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Djian P
Centre National de la Recherche Scientifique, UPR 2228, Régulation de la Transcription et Maladies Génétiques, Université René Descartes, 75270 Paris cedex 06, France. djian@biomedicale.univ-paris5.fr
Easley K
Green H
References (37)
37 references, click to expand
  1. Expansion of mouse involucrin by intra-allelic repeat addition.
    Gene. 2000 Jul 11;252(1-2):195-207 PMID: 10903451
  2. Inability of keratinocytes lacking their specific transglutaminase to form cross-linked envelopes: absence of envelopes as a simple diagnostic test for lamellar ichthyosis.
    Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):687-90 PMID: 9435253
  3. Lessons from loricrin-deficient mice: compensatory mechanisms maintaining skin barrier function in the absence of a major cornified envelope protein.
    J Cell Biol. 2000 Oct 16;151(2):389-400 PMID: 11038185
  4. Relation of protein synthesis and transglutaminase activity to formation of the cross-linked envelope during terminal differentiation of the cultured human epidermal keratinocyte.
    J Cell Biol. 1978 Mar;76(3):705-11 PMID: 24643
  5. Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions.
    Cell. 1979 Nov;18(3):681-94 PMID: 42494
  6. Involucrin synthesis and tissue assembly by keratinocytes in natural and cultured human epithelia.
    J Cell Biol. 1981 Sep;90(3):732-7 PMID: 6895225
  7. Participation of membrane-associated proteins in the formation of the cross-linked envelope of the keratinocyte.
    Cell. 1984 Apr;36(4):827-34 PMID: 6200236
  8. Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro.
    Cell. 1985 Mar;40(3):677-83 PMID: 2578890
  9. Keratinocyte-specific transglutaminase of cultured human epidermal cells: relation to cross-linked envelope formation and terminal differentiation.
    Cell. 1985 Mar;40(3):685-95 PMID: 2578891
  10. Involucrin acts as a transglutaminase substrate at multiple sites.
    Biochem Biophys Res Commun. 1986 Apr 14;136(1):51-6 PMID: 2871834
  11. Structure and evolution of the human involucrin gene.
    Cell. 1986 Aug 15;46(4):583-9 PMID: 2873896
  12. Covalently bound omega-hydroxyacylsphingosine in the stratum corneum.
    Biochim Biophys Acta. 1987 Jan 13;917(1):108-11 PMID: 3790600
  13. Evidence that the corneocyte has a chemically bound lipid envelope.
    J Invest Dermatol. 1987 Jun;88(6):709-13 PMID: 3585054
  14. Isolation, characterization, and UV-stimulated expression of two families of genes encoding polypeptides of related structure in human epidermal keratinocytes.
    Mol Cell Biol. 1988 May;8(5):2195-203 PMID: 3133554
  15. Remodeling of the involucrin gene during primate evolution.
    Cell. 1988 Aug 12;54(4):491-6 PMID: 3401924
  16. The involucrin gene of the galago. Existence of a correction process acting on its segment of repeats.
    J Biol Chem. 1990 May 15;265(14):7804-7 PMID: 2335506
  17. Identification of a major keratinocyte cell envelope protein, loricrin.
    Cell. 1990 Jun 15;61(6):1103-12 PMID: 2190691
  18. The involucrin genes of pig and dog: comparison of their segments of repeats with those of prosimians and higher primates.
    Mol Biol Evol. 1990 Jul;7(4):293-302 PMID: 2385171
  19. Involucrin gene of tarsioids and other primates: alternatives in evolution of the segment of repeats.
    Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5321-5 PMID: 1905021
  20. Simplified mammalian DNA isolation procedure.
    Nucleic Acids Res. 1991 Aug 11;19(15):4293 PMID: 1870982
  21. Biophysical characterization of involucrin reveals a molecule ideally suited to function as an intermolecular cross-bridge of the keratinocyte cornified envelope.
    J Biol Chem. 1992 Jun 15;267(17):12233-8 PMID: 1601889
  22. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.
    Cell. 1992 Jun 12;69(6):915-26 PMID: 1606615
  23. Evidence that involucrin is a covalently linked constituent of highly purified cultured keratinocyte cornified envelopes.
    J Invest Dermatol. 1993 Jan;100(1):3-9 PMID: 8423390
  24. Involucrin--structure and role in envelope assembly.
    J Invest Dermatol. 1993 May;100(5):613-7 PMID: 8098344
  25. Tissue-specific and differentiation-appropriate expression of the human involucrin gene in transgenic mice: an abnormal epidermal phenotype.
    Differentiation. 1993 Jul;53(3):191-200 PMID: 8405770
  26. Ceramides are bound to structural proteins of the human foreskin epidermal cornified cell envelope.
    J Biol Chem. 1998 Jul 10;273(28):17763-70 PMID: 9651377
  27. Transglutaminase action imitates Huntington's disease: selective polymerization of Huntingtin containing expanded polyglutamine.
    Mol Cell. 1998 Mar;1(4):595-601 PMID: 9660943
  28. The involucrin genes of the mouse and the rat: study of their shared repeats.
    Mol Biol Evol. 1993 Nov;10(6):1136-49 PMID: 8277848
  29. Mutations of keratinocyte transglutaminase in lamellar ichthyosis.
    Science. 1995 Jan 27;267(5197):525-8 PMID: 7824952
  30. Sequence and expression patterns of mouse SPR1: Correlation of expression with epithelial function.
    J Invest Dermatol. 1996 Feb;106(2):294-304 PMID: 8601731
  31. A molecular defect in loricrin, the major component of the cornified cell envelope, underlies Vohwinkel's syndrome.
    Nat Genet. 1996 May;13(1):70-7 PMID: 8673107
  32. Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin.
    J Cell Biol. 1996 Aug;134(3):715-29 PMID: 8707850
  33. Direct evidence that involucrin is a major early isopeptide cross-linked component of the keratinocyte cornified cell envelope.
    J Biol Chem. 1997 Jan 17;272(3):2021-30 PMID: 8999895
  34. The involucrin gene of the tree shrew: recent repeat additions and the relocation of cysteine codons.
    Gene. 1997 Mar 10;187(1):29-34 PMID: 9073063
  35. S100A11, S100A10, annexin I, desmosomal proteins, small proline-rich proteins, plasminogen activator inhibitor-2, and involucrin are components of the cornified envelope of cultured human epidermal keratinocytes.
    J Biol Chem. 1997 May 2;272(18):12035-46 PMID: 9115270
  36. Periplakin, a novel component of cornified envelopes and desmosomes that belongs to the plakin family and forms complexes with envoplakin.
    J Cell Biol. 1997 Dec 29;139(7):1835-49 PMID: 9412476
  37. Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase).
    Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1044-9 PMID: 9448282
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.151.2.381
Email
djian@biomedicale.univ-paris5.fr
Published
2000-10-16
Pages
381-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2192633
Subset
IM
Corrections
CommentIn
PMID: 11038193
External Links
PubMed source DOI: 10.1083/jcb.151.2.381 PMC Full Text View journal details
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