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PMID: 11035813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The semaphorin receptor plexin-B1 specifically interacts with active Rac in a ligand-dependent manner.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 23 ·2000-11-07 ·Pages 12457-62

Vikis HG, Li W, He Z, Guan KL

Abstract

Semaphorin molecules serve as axon guidance signals that regulate the navigation of neuronal growth cones. Semaphorins have also been implicated in other biological processes, including the immune response. Plexins, acting either alone or in complex with neuropilins, have recently been identified as functional semaphorin receptors. However, the mechanisms of signal transduction by plexins remain largely unknown. We have demonstrated a direct interaction between plexin-B1 and activated Rac. Rac specifically interacts with the cytosolic domain of plexin-B1, but not with that of plexin-A3 or -C1. Neither RhoA nor Cdc42 interacts with plexin-B1, indicating that the Rac/plexin-B1 interaction is highly specific. The binding of GTP and the integrity of the Rac effector domain are required for the interaction with plexin-B1. Furthermore, we have identified that a Cdc42/Rac interactive binding (CRIB) motif in the cytosolic domain of plexin-B1 is essential for its interaction with active Rac. We have also observed that the semaphorin CD100, a ligand for plexin-B1, stimulates the interaction between plexin-B1 and active Rac. Our results support a model by which activated Rac plays a role in mediating semaphorin signals, resulting in reorganization of actin cytoskeletal structure.

MeSH Terms
Amino Acid Motifs Antigens, CD Binding Sites Cell Line Humans Ligands Membrane Glycoproteins/metabolism Nerve Tissue Proteins/chemistry,metabolism Receptors, Cell Surface/chemistry,metabolism Semaphorins Structure-Activity Relationship rac1 GTP-Binding Protein/metabolism
Chemicals
Antigens, CD CD100 antigen Ligands Membrane Glycoproteins Nerve Tissue Proteins PLXNB1 protein, human Receptors, Cell Surface Semaphorins rac1 GTP-Binding Protein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vikis H G
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Li W
He Z
Guan K L
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.220421797
Published
2000-11-07
Pages
12457-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC18785
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.220421797 PMC Full Text View journal details
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