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PMID: 11032321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Common methylation characteristics of sex chromosomes in somatic and germ cells from mouse, lemur and human.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology ·Vol. 8 ·No. 6 ·2000-00-00 ·Pages 513-25

Bernardino J, Lombard M, Niveleau A, Dutrillaux B

Abstract

DNA methylation of sex chromosomes was analysed using anti-5-methylcytosine antibodies on metaphase chromosomes of somatic cells from three species: human, lemur and mouse. Germ cells were also studied in male mouse. In female cells (human and mouse), the late replicating X was always the less methylated chromosome. Compared with autosomes, the methylation of both X chromosomes was always lower in fibroblasts than in lymphocytes and the difference was always greater in mouse than in human. In human, mouse and lemur male cells, the labelling of the unique X chromosome was quite similar to that of the early replicating X from female cells. Except for the heterochromatic region of the human Y chromosome, strongly methylated, the overall methylation of the Y chromosome was low. In mouse testicular cells, a variety of DNA methylation patterns was observed according to the cell type and the state of differentiation. Finally, the only structures of sex chromosomes which remain methylated in all conditions correspond to their pseudoautosomal regions.

MeSH Terms
Animals Bone Marrow Cells/cytology Cells, Cultured DNA Methylation Female Fibroblasts/cytology Germ Cells/cytology,metabolism Humans Lemur Lymphocytes/cytology Male Meiosis Mice Sertoli Cells/cytology X Chromosome Y Chromosome
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bernardino J
Laboratoire d'étude de la Radiosensibilité des Cellules Germinales, Département de Radiobiologie et Radiopathologie, Fontenay-aux-roses, France.
Lombard M
Niveleau A
Dutrillaux B
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Article Info
Journal
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Abbr.
Chromosome Res
ISSN
0967-3849
DOI
10.1023/a:1009271706488
Published
2000-00-00
Pages
513-25
Language
English
Region
Netherlands
NLM ID
9313452
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1009271706488 View journal details
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