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题目:: Pax7 is required for the specification of myogenic satellite cells.
作者:: Seale(P),Sabourin(L A),Girgis-Gabardo(A),Mansouri(A),Gruss(P),Rudnicki(M A)
状态:: 发布时间2000-10-30 , 更新时间 2008-11-21
期刊:: Cell
摘要:: The paired box transcription factor Pax7 was isolated by representational difference analysis as a gene specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization revealed that Pax7 was also expressed in satellite cells residing in adult muscle. Cell culture and electron microscopic analysis revealed a complete absence of satellite cells in Pax7(-/-) skeletal muscle. Surprisingly, fluorescence-activated cell sorting analysis indicated that the proportion of muscle-derived stem cells was unaffected. Importantly, stem cells from Pax7(-/-) muscle displayed almost a 10-fold increase in their ability to form hematopoietic colonies. These results demonstrate that satellite cells and muscle-derived stem cells represent distinct cell populations. Together these studies suggest that induction of Pax7 in muscle-derived stem cells induces satellite cell specification by restricting alternate developmental programs.
语言:: eng
DOI:

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