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PMID: 11024361 Published · ppublish English Journal Article Review

The use of recombinant antibodies in proteomics.

Current opinion in biotechnology ·Vol. 11 ·No. 5 ·2000-10-00 ·Pages 445-9

Holt LJ, Enever C, de Wildt RM, Tomlinson IM

Abstract

Recombinant antibodies are becoming increasingly important in the field of proteomics. Recent advances include the development of large phage-antibody libraries that contain high-affinity binders to almost any target protein, and new methods for high-throughput selection of antibody-antigen interactions. Coupled with a range of new screening technologies that use high-density antibody arrays to identify differentially expressed proteins, these antibody libraries can be applied to whole proteome analysis.

MeSH Terms
Antibodies, Monoclonal/genetics,immunology Gene Expression Profiling/methods Genetic Vectors Proteome/immunology Recombinant Proteins/immunology
Chemicals
Antibodies, Monoclonal Proteome Recombinant Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Holt L J
MRC Laboratory of Molecular Biology and MRC Centre for Protein Engineering, Hills Road, CB2 2QH, Cambridge, UK.
Enever C
de Wildt R M
Tomlinson I M
Article Info
Journal
Current opinion in biotechnology
Abbr.
Curr Opin Biotechnol
ISSN
0958-1669
Published
2000-10-00
Pages
445-9
Language
English
Region
England
NLM ID
9100492
Subset
IM
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