Specificity of deoxyribonucleic acid transmethylase induced by bacteriophage T2. I. Nucleotide sequences isolated from tmicrococcus luteus DNA methylated in vitro.

Nucleic acids research ·Vol. 2 ·No. 8 ·1975-08-00 ·Pages 1391-400

van Ormondt H, Gorter J, Havelaar KJ, de Waard A

Abstract

(Deoxyribonucleic acid from Micrococcus luteus was methylated in vitro in the presence of S-adenosyl-(14C methyl)methionine with a DNA methyltransferase purified from extracts of te. coli infected with bacteriophage T2. The labelled DNA was degraded by enzymatic and specific chemical methods and the resulting short oligonucleotides were separated and characterized. tthe analytical data permit the conclusion that the tdna transmethylase reacts specifically with N-G-A-T-C-N sequences in which it converts adenine to a 6-methyl-aminopurine residue.

MeSH Terms

Coliphages/enzymology DNA (Cytosine-5-)-Methyltransferases/isolation & purification,metabolism DNA Viruses/enzymology DNA, Bacterial Deoxyribonucleases Electrophoresis, Polyacrylamide Gel Enzyme Induction Escherichia coli/enzymology Exonucleases Methyltransferases/metabolism Micrococcus/metabolism Oligonucleotides/analysis S-Adenosylmethionine/metabolism

Chemicals

DNA, Bacterial Oligonucleotides S-Adenosylmethionine Methyltransferases DNA (Cytosine-5-)-Methyltransferases Deoxyribonucleases Exonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

van Ormondt H

Gorter J

Havelaar K J

de Waard A

References (9)

9 references, click to expand

THE ENZYMATIC METHYLATION OF RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID. V. PURIFICATION AND PROPERTIES OF THE DEOXYRIBONUCLEIC ACID-METHYLATING ACTIVITY OF ESCHERICHIA COLI.
J Biol Chem. 1964 Nov;239:3858-65 PMID: 14257620
THE ENZYMATIC METHYLATION OF RNA AND DNA. 8. EFFECTS OF BACTERIOPHAGE INFECTION ON THE ACTIVITY OF THE METHYLATING ENZYMES.
Proc Natl Acad Sci U S A. 1964 Aug;52:292-7 PMID: 14206593
A lipopolysaccharide inhibitor of a DNA methyl transferase.
Proc Natl Acad Sci U S A. 1965 Dec;54(6):1713-20 PMID: 4286564
Methylated oligonucleotides derived from bacteriophage fd RF-DNA modified in vitro by E. coli B modification methylase.
FEBS Lett. 1973 Jul 1;33(2):177-80 PMID: 4580897
Nucleotide 'maps' of digests of deoxyribonucleic acid.
Biochem J. 1970 Aug;118(5):831-41 PMID: 5476726
Mutants of bacteriophage T2 gt with altered DNA methylase activity.
J Mol Biol. 1972 Jun 28;67(3):351-60 PMID: 5045302
Mutants of T2gt with altered DNA methylase activity: relation to restriction by prophage P1.
Virology. 1971 Aug;45(2):484-95 PMID: 4937994
The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IX. Deoxyribonucleic acid methylase in bacteriophage-infected Escherichia coli.
J Biol Chem. 1966 May 10;241(9):1985-94 PMID: 5329749
Nucleotide sequences at the sites of action of the deoxyribonucleic acid modification enzyme of bacteriophage P1.
J Mol Biol. 1974 Sep 15;88(2):437-43 PMID: 4453003

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/2.8.1391

Published

1975-08-00

Pages

1391-400

Language

English

Region

England

NLM ID

0411011

PMCID

PMC344390

Subset

External Links

PubMed source DOI: 10.1093/nar/2.8.1391 PMC Full Text View journal details

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Specificity of deoxyribonucleic acid transmethylase induced by bacteriophage T2. I. Nucleotide sequences isolated from tmicrococcus luteus DNA methylated in vitro.

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