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PMID: 10999411 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Systemic silencing signal(s).

Plant molecular biology ·Vol. 43 ·No. 2-3 ·2000-06-00 ·Pages 285-93

Fagard M, Vaucheret H

Abstract

Grafting experiments have revealed that transgenic plants that undergo co-suppression of homologous transgenes and endogenous genes or PTGS of exogenous transgenes produce a sequence-specific systemic silencing signal that is able to propagate from cell to cell and at long distance. Similarly, infection of transgenic plants by viruses that carry (part of) a transgene sequence results in global silencing (VIGS) of the integrated transgenes although viral infection is localized. Systemic PTGS and VIGS strongly resemble recovery from virus infection in non-transgenic plants, leading to protection against secondary infection in newly emerging leaves and PTGS of transiently expressed homologous transgenes. The sequence-specific PTGS signal is probably a transgene product (for example, aberrant RNA) or a secondary product (for example, RNA molecules produced by an RNA-dependent RNA polymerase with transgene RNA as a matrix) that mimics the type of viral RNA that is targeted for degradation by cellular defence. Whether some particular cases of transgene TGS could also rely on the production of such a mobile molecule is discussed.

MeSH Terms
Gene Expression Regulation, Plant/genetics,physiology Gene Silencing/physiology RNA Processing, Post-Transcriptional Signal Transduction
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fagard M
Laboratoire de Biologie Cellulaire, INRA, Versailles, France.
Vaucheret H
References (47)
47 references, click to expand
  1. An RNA-based information superhighway in plants.
    Science. 1998 Mar 6;279(5356):1486-7 PMID: 9508725
  2. Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase.
    Science. 1999 Dec 17;286(5448):2342-4 PMID: 10600745
  3. Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes.
    Plant Cell. 1996 Feb;8(2):179-188 PMID: 12239381
  4. Genetics of homology-dependent gene silencing in Arabidopsis; a role for methylation.
    Plant J. 1997 Oct;12(4):791-804 PMID: 9375393
  5. A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing.
    Cell. 1998 Nov 13;95(4):461-70 PMID: 9827799
  6. Differences in DNA-methylation are associated with a paramutation phenomenon in transgenic petunia.
    Plant J. 1993 Jul;4(1):89-100 PMID: 8220478
  7. Sequence homology requirements for transcriptional silencing of 35S transgenes and post-transcriptional silencing of nitrite reductase (trans)genes by the tobacco 271 locus.
    Plant Mol Biol. 1996 Dec;32(6):1075-83 PMID: 9002606
  8. Transgenes are dispensable for the RNA degradation step of cosuppression.
    Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9675-80 PMID: 9689140
  9. A similarity between viral defense and gene silencing in plants.
    Science. 1997 Jun 6;276(5318):1558-60 PMID: 18610513
  10. Homology-dependent gene silencing in transgenic plants: epistatic silencing loci contain multiple copies of methylated transgenes.
    Mol Gen Genet. 1994 Aug 2;244(3):219-29 PMID: 8058033
  11. Transgene-induced gene silencing in plants.
    Plant J. 1998 Dec;16(6):651-9 PMID: 10069073
  12. Phloem Unloading in Sink Leaves of Nicotiana benthamiana: Comparison of a Fluorescent Solute with a Fluorescent Virus.
    Plant Cell. 1997 Aug;9(8):1381-1396 PMID: 12237387
  13. Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans.
    Plant Cell. 1990 Apr;2(4):279-289 PMID: 12354959
  14. Epigenetic control of an endogenous gene family is revealed by a novel blue fluorescent mutant of Arabidopsis.
    Cell. 1995 Dec 1;83(5):725-34 PMID: 8521489
  15. Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.
    Plant Cell. 1990 Apr;2(4):291-9 PMID: 2152117
  16. A viral suppressor of gene silencing in plants.
    Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13079-84 PMID: 9789044
  17. Genome amplification and long-distance movement functions associated with the central domain of tobacco etch potyvirus helper component-proteinase.
    Virology. 1997 Feb 17;228(2):251-62 PMID: 9123832
  18. How and Why Do Plants Inactivate Homologous (Trans)genes?
    Plant Physiol. 1995 Mar;107(3):679-685 PMID: 12228391
  19. Transcriptional and posttranscriptional plant gene silencing in response to a pathogen
    Science. 1998 Mar 27;279(5359):2113-5 PMID: 9516113
  20. Listening to the silent genes: transgene silencing, gene regulation and pathogen control.
    Trends Plant Sci. 1999 Sep;4(9):340-347 PMID: 10462766
  21. Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants.
    EMBO J. 1989 Mar;8(3):643-9 PMID: 16453872
  22. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA.
    Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13959-64 PMID: 9811908
  23. An Arabidopsis mutant with a reduced level of cab140 RNA is a result of cosuppression.
    Plant Cell. 1993 Jun;5(6):667-77 PMID: 8329898
  24. RNA-mediated RNA degradation and chalcone synthase A silencing in petunia.
    Cell. 1997 Mar 21;88(6):845-54 PMID: 9118227
  25. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA.
    Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1679-83 PMID: 7878039
  26. A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement.
    EMBO J. 1995 Dec 1;14(23):5762-72 PMID: 8846770
  27. Distinct phenotypes generated by overexpression and suppression of S-adenosyl-L-methionine synthetase reveal developmental patterns of gene silencing in tobacco.
    Plant Cell. 1994 Oct;6(10):1401-14 PMID: 7994174
  28. DNA endoreduplication and polyteny understood as evolutionary strategies.
    Nature. 1976 Jun 17;261(5561):614-5 PMID: 934306
  29. Activation of systemic acquired silencing by localised introduction of DNA.
    Curr Biol. 1999 Jan 28;9(2):59-66 PMID: 10021363
  30. A species of small antisense RNA in posttranscriptional gene silencing in plants.
    Science. 1999 Oct 29;286(5441):950-2 PMID: 10542148
  31. Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA.
    Cell. 1998 Oct 16;95(2):177-87 PMID: 9790525
  32. Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions.
    EMBO J. 1997 Aug 1;16(15):4738-45 PMID: 9303318
  33. Production of aberrant promoter transcripts contributes to methylation and silencing of unlinked homologous promoters in trans.
    EMBO J. 1999 Jan 4;18(1):241-8 PMID: 9878066
  34. Systemic signalling in gene silencing.
    Nature. 1997 Oct 9;389(6651):553 PMID: 9335491
  35. Infection of tobacco or Arabidopsis plants by CMV counteracts systemic post-transcriptional silencing of nonviral (trans)genes.
    Virology. 1998 Dec 20;252(2):313-7 PMID: 9878609
  36. Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana.
    EMBO J. 1998 Nov 16;17(22):6739-46 PMID: 9822616
  37. Frequencies, Timing, and Spatial Patterns of Co-Suppression of Nitrate Reductase and Nitrite Reductase in Transgenic Tobacco Plants.
    Plant Physiol. 1996 Dec;112(4):1447-1456 PMID: 12226457
  38. Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance.
    Plant Cell. 1993 Dec;5(12):1749-1759 PMID: 12271055
  39. RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing.
    Plant Cell. 1999 Dec;11(12):2291-301 PMID: 10590159
  40. Initiation and maintenance of virus-induced gene silencing
    Plant Cell. 1998 Jun;10(6):937-46 PMID: 9634582
  41. An inverted repeat triggers cytosine methylation of identical sequences in Arabidopsis.
    Mol Cell. 1999 Apr;3(4):505-11 PMID: 10230403
  42. Gene silencing without DNA. rna-mediated cross-protection between viruses
    Plant Cell. 1999 Jul;11(7):1207-16 PMID: 10402423
  43. Gene silencing from plant DNA carried by a Geminivirus.
    Plant J. 1998 Apr;14(1):91-100 PMID: 15494056
  44. Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase.
    Nature. 1999 May 13;399(6732):166-9 PMID: 10335848
  45. A Transcriptionally Active State Is Required for Post-Transcriptional Silencing (Cosuppression) of Nitrate Reductase Host Genes and Transgenes.
    Plant Cell. 1997 Aug;9(8):1495-1504 PMID: 12237392
  46. RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants.
    Plant Mol Biol. 1996 Oct;32(1-2):79-88 PMID: 8980475
  47. A model for RNA-mediated gene silencing in higher plants.
    Plant Mol Biol. 1998 May;37(2):349-62 PMID: 9617806
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1006404016494
Published
2000-06-00
Pages
285-93
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1006404016494 View journal details
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