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PMID: 1098730 Published · ppublish English Comparative Study Journal Article

Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis.

The British journal of venereal diseases ·Vol. 51 ·No. 4 ·1975-08-00 ·Pages 227-31

Veldkamp J, Visser AM

Abstract

The enzyme-linked immunosorbent assay (ELISA) technique, using an ultrasonicate of Treponema pallidum as antigen, has been evaluated as a serological test for syphilis. It is concluded that the test is simple, reliable, and relatively quick and that its sensitivity in all stages of syphilis is equal to the FTAABS test. Because its specificity is probably also high, ELISA might be used in future as a first-line screening test for the serodiagnosis of syphilis.

MeSH Terms
Adsorption Alkaline Phosphatase Antibodies, Anti-Idiotypic Complement Fixation Tests Fluorescent Antibody Technique Humans Immunoglobulins Syphilis Serodiagnosis/methods Treponema Immobilization Test
Chemicals
Antibodies, Anti-Idiotypic Immunoglobulins Alkaline Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Veldkamp J
Visser A M
References (6)
6 references, click to expand
  1. Performance and use of the Reiter protein complement-fixation (RPCF) test.
    Br J Vener Dis. 1966 Mar;42(1):42-3 PMID: 5929698
  2. Experiences with the treponemal immobilization test in the Netherlands.
    Antonie Van Leeuwenhoek. 1955;21(3):331-6 PMID: 13249379
  3. The antigenic structure of Treponema pallidum, Nichols strain. I. The demonstration, nature and location of specific and shared antigens.
    J Immunol. 1966 Mar;96(3):450-6 PMID: 5326762
  4. Isolation and purification of the protein antigen of the Reiter treponeme; a study of its serologic reactions.
    Am J Syph Gonorrhea Vener Dis. 1953 Mar;37(2):137-50 PMID: 13030987
  5. Immunoassay using antigen-enzyme conjugates.
    FEBS Lett. 1971 Jun 24;15(3):232-236 PMID: 11945853
  6. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.
    Immunochemistry. 1971 Sep;8(9):871-4 PMID: 5135623
Article Info
Journal
The British journal of venereal diseases
Abbr.
Br J Vener Dis
ISSN
0007-134X
Published
1975-08-00
Pages
227-31
Language
English
Region
England
NLM ID
0421042
PMCID
PMC1046555
Subset
IM
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