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PMID: 10986270 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Bacillus subtilis ccpA gene mutants specifically defective in activation of acetoin biosynthesis.

Journal of bacteriology ·Vol. 182 ·No. 19 ·2000-10-00 ·Pages 5611-4

Turinsky AJ, Moir-Blais TR, Grundy FJ, Henkin TM

Abstract

A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is involved in acetoin biosynthesis, were identified. These mutants retained normal glucose repression of amyE, encoding alpha-amylase, and acsA, encoding acetyl-coenzyme A synthetase, and normal activation of ackA, which is involved in acetate excretion; in these ccpA mutants the CcpA functions of activation of the acetate and acetoin excretion pathways appear to be separated.

MeSH Terms
Acetates/metabolism Acetoin/metabolism Acetolactate Synthase/genetics,metabolism Bacillus subtilis/genetics,growth & development,metabolism Bacterial Proteins Carboxy-Lyases/genetics,metabolism Culture Media DNA-Binding Proteins/genetics,metabolism Gene Expression Genes, Bacterial Hydrogen-Ion Concentration Lac Operon Mutagenesis Repressor Proteins/genetics,metabolism Transcription, Genetic
Chemicals
Acetates Bacterial Proteins Culture Media DNA-Binding Proteins Repressor Proteins catabolite control proteins, bacteria Acetoin Acetolactate Synthase Carboxy-Lyases acetolactate decarboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Turinsky A J
Department of Biochemistry and Molecular Biology, Albany Medical College, Albany, New York 12208, USA.
Moir-Blais T R
Grundy F J
Henkin T M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.19.5611-5614.2000
Published
2000-10-00
Pages
5611-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC111010
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.182.19.5611-5614.2000 PMC Full Text View journal details
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