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PMID: 10984455 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparative maps of human 19p13.3 and mouse chromosome 10 allow identification of sequences at evolutionary breakpoints.

Genome research ·Vol. 10 ·No. 9 ·2000-09-00 ·Pages 1369-80

Puttagunta R, Gordon LA, Meyer GE, Kapfhamer D, Lamerdin JE, Kantheti P, Portman KM, Chung WK, Jenne DE, Olsen AS, Burmeister M

Abstract

A cosmid/bacterial artificial chromosome (BAC) contiguous (contig) map of human chromosome (HSA) 19p13.3 has been constructed, and over 50 genes have been localized to the contig. Genes and anonymous ESTs from approximately 4000 kb of human 19p13.3 were placed on the central mouse chromosome 10 map by genetic mapping and pulsed-field gel electrophoresis (PFGE) analysis. A region of approximately 2500 kb of HSA 19p13.3 is collinear to mouse chromosome (MMU) 10. In contrast, the adjacent approximately 1200 kb are inverted. Two genes are located in a 50-kb region after the inversion on MMU 10, followed by a region of homology to mouse chromosome 17. The synteny breakpoint and one of the inversion breakpoints has been localized to sequenced regions in human <5 kb in size. Both breakpoints are rich in simple tandem repeats, including (TCTG)n, (CT)n, and (GTCTCT)n, suggesting that simple repeat sequences may be involved in chromosome breaks during evolution. The overall size of the region in mouse is smaller, although no large regions are missing. Comparing the physical maps to the genetic maps showed that in contrast to the higher-than-average rate of genetic recombination in gene-rich telomeric region on HSA 19p13.3, the average rate of recombination is lower than expected in the homologous mouse region. This might indicate that a hot spot of recombination may have been lost in mouse or gained in human during evolution, or that the position of sequences along the chromosome (telomeric compared to the middle of a chromosome) is important for recombination rates.

MeSH Terms
Animals Chromosome Breakage/genetics Chromosome Inversion Chromosomes, Bacterial/genetics Chromosomes, Human, Pair 19/genetics Cosmids/genetics Electrophoresis, Gel, Pulsed-Field Evolution, Molecular Female Genetic Markers/genetics Humans Male Mice Mice, Inbred C57BL Mice, Neurologic Mutants Physical Chromosome Mapping Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid
Chemicals
Genetic Markers
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Puttagunta R
Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.
Gordon L A
Meyer G E
Kapfhamer D
Lamerdin J E
Kantheti P
Portman K M
Chung W K
Jenne D E
Olsen A S
Burmeister M
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.145200
Published
2000-09-00
Pages
1369-80
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC310909
Subset
IM
Grants
NINDS NIH HHS · R01 NS032130 · United States
NINDS NIH HHS · NS32130 · United States
External Links
PubMed source DOI: 10.1101/gr.145200 PMC Full Text View journal details
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