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PMID: 109810 Published · ppublish English Journal Article

Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA.

Nucleic acids research ·Vol. 6 ·No. 4 ·1979-04-00 ·Pages 1287-308

Guilley H, Jonard G, Kukla B, Richards KE

Abstract

The sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA has been determined. The sequence contains the entire coat protein cistron as well as regions to its left and right. Sequence characterization was by conventional methods for use with uniformly 32P labeled RNA complemented by newer methods for in vitro 5' and 3' 32P end-labeling of RNA and its subsequent rapid analysis. The noncoding region separating the coat protein cistron from the 3' terminus is 204 residues long and may be folded into a clover-leaf-type secondary structure. The distribution of termination codons to the left of the coat protein cistron suggests that the end of the adjacent cistron is separated from the beginning of the coat protein cistron by only two nucleotides. The subgenomic viral coat protein mRNA was isolated from infected tissue and shown to be capped. The nontranslated sequence separating the cap from the AUG initiation codon is 9 residues long and thus overlaps a portion of the adjacent cistron on the genome RNA.

MeSH Terms
Base Sequence Genetic Code Molecular Weight RNA, Messenger/analysis RNA, Viral Ribonuclease T1 Tobacco Mosaic Virus/analysis Viral Proteins/biosynthesis
Chemicals
RNA, Messenger RNA, Viral Viral Proteins Ribonuclease T1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guilley H
Jonard G
Kukla B
Richards K E
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41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.4.1287
Published
1979-04-00
Pages
1287-308
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327773
Subset
IM
Databases
GENBANK
J02415
External Links
PubMed source DOI: 10.1093/nar/6.4.1287 PMC Full Text View journal details
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