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PMID: 10980121 Published · ppublish English Journal Article

Up-regulation of galectin-3 in acute renal failure of the rat.

The American journal of pathology ·Vol. 157 ·No. 3 ·2000-09-00 ·Pages 815-23

Nishiyama J, Kobayashi S, Ishida A, Nakabayashi I, Tajima O, Miura S, Katayama M, Nogami H

Abstract

Galectin-3, a multifunctional beta-galactoside-binding lectin, is known to participate in development, oncogenesis, cell-to-cell attachment, and inflammation. We studied to determine whether galectin-3 is associated with cell injury and regeneration in two types of acute renal failure (ARF), namely ischemic and toxic ARF. In ischemia/reperfusion renal injury in rats (bilateral renal pedicles clamped for 40 minutes), galectin-3 mRNA began to increase at 2 hours and extended by 6.2-fold at 48 hours (P: < 0.01 versus normal control rats), and then decreased by 28 days after injury. In addition, a significant negative correlation between galectin-3 mRNA expression and serum reciprocal creatinine was shown at 48 hours after injury (n = 13, r = -0.94, P: < 0.0001). In folic acid-induced ARF, galectin-3 mRNA was found to be up-regulated at 2 hours after injury and increased levels continued until at least 7 days post-injury. In immunohistochemistry, at 2 hours following reperfusion, galectin-3 began to develop in proximal convoluted tubules. From 6 hours up to 48 hours, galectin-3 was also found in proximal straight tubules, distal tubules, thick ascending limbs, and collecting ducts. In later stages of regeneration, galectin-3 expressions were found in macrophages. In conclusion, we demonstrated that galectin-3 expressions were markedly up-regulated in both ischemic and toxic types of ARF. Galectin-3 may play an important role in acute tubular injury and the following regeneration stage.

MeSH Terms
Acute Kidney Injury/chemically induced,metabolism,pathology Animals Antigens, Differentiation/genetics,metabolism Creatinine/blood Folic Acid/toxicity Galectin 3 Immunoenzyme Techniques Kidney/drug effects,pathology,surgery Lectins/genetics,metabolism Male RNA, Messenger/metabolism Rats Rats, Sprague-Dawley Reperfusion Injury/metabolism,pathology Up-Regulation
Chemicals
Antigens, Differentiation Galectin 3 Lectins RNA, Messenger Folic Acid Creatinine
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Nishiyama J
Second Department of Medicine, National Defense Medical College, Tokorozawa, Japan.
Kobayashi S
Ishida A
Nakabayashi I
Tajima O
Miura S
Katayama M
Nogami H
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)64595-6
Published
2000-09-00
Pages
815-23
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1885699
Subset
IM
External Links
PubMed source DOI: 10.1016/S0002-9440(10)64595-6 PMC Full Text View journal details
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