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PMID: 10970846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cbk1p, a protein similar to the human myotonic dystrophy kinase, is essential for normal morphogenesis in Saccharomyces cerevisiae.

The EMBO journal ·Vol. 19 ·No. 17 ·2000-09-01 ·Pages 4524-32

Racki WJ, Bécam AM, Nasr F, Herbert CJ

Abstract

We have studied the CBK1 gene of Saccharomyces cerevisiae, which encodes a conserved protein kinase similar to the human myotonic dystrophy kinase. We have shown that the subcellular localization of the protein, Cbk1p, varies in a cell cycle-dependent manner. Three phenotypes are associated with the inactivation of the CBK1 gene: large aggregates of cells, round rather than ellipsoidal cells and a change from a bipolar to a random budding pattern. Two-hybrid and extragenic suppressor studies have linked Cbk1p with the transcription factor Ace2p, which is responsible for the transcription of chitinase. Cbk1p is necessary for the activation of Ace2p and we have shown that the aggregation phenotype is due to a lack of chitinase expression. The random budding pattern and the round cell phenotype of the CBK1 deletion strain show that in addition to its role in regulating chitinase expression via Ace2p, Cbk1p is essential for a wild-type morphological development of the cell.

MeSH Terms
Base Sequence Chitinases/genetics DNA Primers DNA-Binding Proteins/genetics Fungal Proteins/genetics,metabolism Gene Deletion Genes, Suppressor Humans Morphogenesis Myotonin-Protein Kinase Protein Serine-Threonine Kinases/metabolism Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins Subcellular Fractions/metabolism Transcription Factors/genetics Transcription, Genetic Two-Hybrid System Techniques
Chemicals
ACE2 protein, S cerevisiae DMPK protein, human DNA Primers DNA-Binding Proteins Fungal Proteins Saccharomyces cerevisiae Proteins Transcription Factors Myotonin-Protein Kinase Protein Serine-Threonine Kinases CTS1 protein, S cerevisiae Chitinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Racki W J
Centre de Génétique Moléculaire, Laboratoire propre du CNRS, Associé à l'Université Pierre et Marie Curie, F-91198, Gif-sur-Yvette, France.
Bécam A M
Nasr F
Herbert C J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.17.4524
Published
2000-09-01
Pages
4524-32
Language
English
Region
England
NLM ID
8208664
PMCID
PMC302079
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.17.4524 PMC Full Text View journal details
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