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PMID: 10970773 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Coupling between inositol 1,4,5-trisphosphate receptors and human transient receptor potential channel 1 when intracellular Ca2+ stores are depleted.

The Biochemical journal ·Vol. 350 Pt 3 ·2000-09-15 ·Pages 631-5

Rosado JA, Sage SO

Abstract

In the present study we have investigated the role of inositol 1,4, 5-trisphosphate (IP(3)), functional IP(3) receptors (IP(3)Rs) and the human homologue of the Drosophila transient receptor potential (Trp) channel, human Trp1 (hTrp1), in store-mediated Ca(2+) entry (SMCE) in human platelets. Inhibition of IP(3) recycling using Li(+), or the inhibition of IP(3)Rs using xestospongin C, both resulted in the inhibition of SMCE activation following Ca(2+) store depletion using thapsigargin. Co-immunoprecipitation experiments indicated that endogenously expressed hTrp1 couples with IP(3)R type II, but not types I or III, in platelets with depleted intracellular Ca(2+) stores, but not in control, undepleted cells. These results provide strong evidence for the activation of SMCE by conformational coupling involving de novo association between IP(3)Rs and a plasma membrane channel in normal human cells.

MeSH Terms
Blood Platelets/metabolism Calcium/metabolism Calcium Channels/metabolism Cells, Cultured Fungal Proteins/metabolism Humans Inositol 1,4,5-Trisphosphate Receptors Protein Binding Protein Isoforms/metabolism Receptors, Cytoplasmic and Nuclear/metabolism
Chemicals
Calcium Channels Fungal Proteins ITPR1 protein, human Inositol 1,4,5-Trisphosphate Receptors Protein Isoforms Receptors, Cytoplasmic and Nuclear Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rosado J A
Department of Physiology, University of Cambridge, Downing Street, Cambridge CB2 3EG, U.K.
Sage S O
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
2000-09-15
Pages
631-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1221291
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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