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PMID: 1095550 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effect of uncouplers on "downhill" beta-galactoside transport in energy-depleted cells of Escherichia coli.

Journal of bacteriology ·Vol. 123 ·No. 1 ·1975-07-00 ·Pages 187-95

Cecchini G, Koch AL

Abstract

Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhydrazone results in stimulation of ONPG transport. The same agents lower transport of non-energy-depleted cells towards the same levels that result from stimulation of the energy depleted cells. Of course, these agents prevent "uphill" accumulation against a concentration gradient under all conditions. Since uncouplers allow normal and energy-depleted cells to assume the same facilitated transport capability, these results lend support to the chemiosmotic hypothesis of Mitchell that comigration of charge is necessary for the transport of neutral galactosides. Our results imply that a potential favorable to transport is maintained by metabolism in non-energy-depleted cells, whereas an unfavorable potential is developed in the initial instant of time when energy-depleted cells are given ONPG.

MeSH Terms

Biological Transport, Active/drug effects Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/pharmacology Cell Membrane/physiology Electrophysiology Escherichia coli/metabolism Formaldehyde/pharmacology Galactosides/metabolism Glycosides/metabolism Membrane Potentials Membrane Transport Modulators Membrane Transport Proteins/antagonists & inhibitors,metabolism Models, Biological Mutation Nitrophenylgalactosides/metabolism Uncoupling Agents/pharmacology

Chemicals

Galactosides Glycosides Membrane Transport Modulators Membrane Transport Proteins Uncoupling Agents Formaldehyde Nitrophenylgalactosides Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Carbonyl Cyanide m-Chlorophenyl Hydrazone

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cecchini G

Koch A L

References (18)

18 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.123.1.187-195.1975

Published

1975-07-00

Pages

187-95

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC235706

Subset

External Links

PubMed source DOI: 10.1128/jb.123.1.187-195.1975 PMC Full Text View journal details

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Effect of uncouplers on "downhill" beta-galactoside transport in energy-depleted cells of Escherichia coli.

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