Home LiteratureArticle Details
PMID: 1095479 Published · ppublish English Journal Article

In vitro lymphocyte response to purified protein derivative, BCG and Mycobacterium leprae in a population not exposed to leprosy.

Infection and immunity ·Vol. 11 ·No. 6 ·1975-06-00 ·Pages 1163-9

Closs O

Abstract

Lymphocytes from 14 BCG-vaccinated donors, seven tuberculin positive and seven tuberculin negative by skin testing, were stimulated in vitro with four mycobacterial antigens, purified protein derivative (PPD), PPD/BCG, whole BCG bacilli, and whole Mycobacterium leprae and also with Candida antigen and phytohemagglutinin. The response was measured by incorporation of (3)H-labeled thymidine. The response to PPD, PPD/BCG, and BCG was found to correlate with the result of skin testing with turberculin. The turberculin-positive group also responded more strongly to M. leprae, whereas the two groups did not differ significantly in their response to Candida antigen or phytohemagglutinin. These findings indicate a certain degree of cross-reactivity between BCG and M. leprae. The use of the lymphocyte transformation test to measure antigenic cross-reactivity is discussed.

MeSH Terms
Adult Antigens, Bacterial Antigens, Fungal BCG Vaccine Candida albicans/immunology Cell Separation Cells, Cultured Cross Reactions Female Humans Lectins Lymphocyte Activation Lymphocytes/immunology Male Mycobacterium bovis Mycobacterium leprae/immunology Thymidine/metabolism Tritium Tuberculin Tuberculin Test
Chemicals
Antigens, Bacterial Antigens, Fungal BCG Vaccine Lectins Tuberculin Tritium Thymidine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Closs O
References (22)
22 references, click to expand
  1. Reactivity of human lymphocytes to aggregated and nonaggregated PPD-tuberculin.
    Cell Immunol. 1972 Dec;5(4):555-60 PMID: 4640815
  2. The response of lymphocytes from tuberculin-positive or negative humans to various doses of PPD-tuberculin in vitro.
    Cell Immunol. 1972 Mar;3(3):493-500 PMID: 5013310
  3. Studies of immune mechanisms in leprosy. 3. The role of cellular and humoral factors in impairment of the in vitro immune response.
    J Immunol. 1971 Apr;106(4):888-99 PMID: 4995005
  4. Transformation of leprous lymphocytes by leprolin, tuberculin and phytohemagglutinin.
    Int J Lepr Other Mycobact Dis. 1971 Oct-Dec;39(4):789-95 PMID: 4949308
  5. Characterization of the cellular immune defect in lepromatous leprosy: a specific lack of circulating Mycobacterium leprae-reactive lymphocytes.
    Clin Exp Immunol. 1971 Dec;9(6):821-31 PMID: 4945738
  6. Immunological phenomena in leprosy and related diseases.
    Adv Immunol. 1971;13:209-66 PMID: 4945362
  7. Lepromin and tuberculin reactivity in adults not exposed to leprosy.
    J Immunol. 1967 Oct;99(4):637-42 PMID: 4860925
  8. Skin hypersensitivity and in vitro lymphocytic reactivity to tuberculin in childhood.
    J Pediatr. 1968 May;72(5):599-605 PMID: 5300556
  9. In vitro lymphocyte responses and skin test reactivity following BCG vaccination.
    Clin Exp Immunol. 1971 Nov;9(5):611-23 PMID: 5145430
  10. Quantitative aspects of the stimulation of lymphocytes by tuberculin purified protein derivative.
    Int Arch Allergy Appl Immunol. 1970;38(2):119-29 PMID: 5411978
  11. Skin sensitivity to human, avian and BCG PPDs after BCG vaccination.
    Tubercle. 1968 Mar;49(1):84-91 PMID: 5690764
  12. Correlation of tuberculin skin reaction with in vitro lymphocyte transformation.
    Am Rev Respir Dis. 1968 May;97(5):904-8 PMID: 5647215
  13. B.C.G. vaccination of children against leprosy in Uganda: results at end of second follow-up.
    Br Med J. 1968 Jan 6;1(5583):24-7 PMID: 5636739
  14. MITOSIS-STIMULATING EFFECT OF TUBERCULIN ON LYMPHOCYTES FROM PERIPHERAL BLOOD.
    J Allergy. 1964 Sep-Oct;35:433-7 PMID: 14216357
  15. Effect of BCG vaccination, lepromin testing and natural causes in inducing reactivity to lepromin and to tuberculin.
    Int J Lepr. 1957 Jan-Mar;25(1):13-37 PMID: 13491128
  16. Comparison of the biologic activity of tuberculins by the use of lymphocyte cultures.
    Am Rev Respir Dis. 1973 Sep;108(3):565-70 PMID: 4745252
  17. PPD tuberculin--a B-cell mitogen.
    Nat New Biol. 1972 Dec 13;240(102):198-200 PMID: 4539345
  18. BCG vaccination of children against leprosy: seven-year findings of the controlled WHO trial in Burma.
    Bull World Health Organ. 1973;48(3):323-34 PMID: 4270384
  19. BCG antibody profiles in tuberculoid and lepromatous leprosy.
    Infect Immun. 1974 May;9(5):952-8 PMID: 4207518
  20. Immunodiffusion analysis--a rational basis for the taxonomy of mycobacteria.
    Ann Soc Belg Med Trop. 1973;53(4):321-30 PMID: 4198566
  21. Immune response to M. leprae of healthy leprosy contacts.
    Int J Lepr Other Mycobact Dis. 1972 Jul-Sep;40(3):243-50 PMID: 4574270
  22. Immunology of tuberculosis in developing countries.
    Ethiop Med J. 1973 Apr;11(2):184 PMID: 4789284
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.11.6.1163-1169.1975
Published
1975-06-00
Pages
1163-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415194
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.11.6.1163-1169.1975 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2