Home LiteratureArticle Details

PMID: 10940046 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The rel gene is essential for in vitro growth of Staphylococcus aureus.

Journal of bacteriology ·Vol. 182 ·No. 17 ·2000-09-00 ·Pages 4995-7

Gentry D, Li T, Rosenberg M, McDevitt D

Abstract

The stringent response in Staphylococcus aureus is mediated by the nucleotide guanosine pentaphosphate, whose synthesis is catalyzed by the product of the rel gene. We report here that the rel gene is essential for the in vitro growth of S. aureus, distinguishing it from all other bacteria tested for this requirement.

MeSH Terms

Genes, Bacterial Ligases/genetics Staphylococcus aureus/enzymology,genetics,growth & development

Chemicals

Ligases guanosine 3',5'-polyphosphate synthetases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gentry D

SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania 19426, USA. dan_r_gentry@sbphrd.com

Li T

Rosenberg M

McDevitt D

References (16)

16 references, click to expand

A rapid test for the rel A mutation in E. coli.
Biochem Biophys Res Commun. 1976 Apr 5;69(3):751-8 PMID: 773380
Cloning and characterization of a bifunctional RelA/SpoT homologue from Mycobacterium tuberculosis.
Gene. 1999 Jun 11;233(1-2):261-9 PMID: 10375643
Cloning the spoT gene of Escherichia coli: identification of the spoT gene product.
J Bacteriol. 1979 Mar;137(3):1100-10 PMID: 374338
Analysis of two chloramphenicol resistance plasmids from Staphylococcus aureus: insertional inactivation of Cm resistance, mapping of restriction sites, and construction of cloning vehicles.
Plasmid. 1981 May;5(3):245-58 PMID: 6267630
Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.
J Biol Chem. 1991 Mar 25;266(9):5980-90 PMID: 2005134
Escherichia coli ppGpp synthetase II activity requires spoT.
J Biol Chem. 1991 Mar 25;266(9):5991-9 PMID: 2005135
Streptomyces ATP nucleotide 3'-pyrophosphokinase and its gene.
Nucleic Acids Symp Ser. 1992;(27):165-6 PMID: 1337783
Occurrence of the regulatory nucleotides ppGpp and pppGpp following induction of the stringent response in staphylococci.
J Bacteriol. 1995 Sep;177(17):5161-5 PMID: 7665499
Functional analysis of a relA/spoT gene homolog from Streptococcus equisimilis.
J Bacteriol. 1996 Mar;178(5):1401-11 PMID: 8631718
Guanosine pentaphosphate synthetase from Streptomyces antibioticus is also a polynucleotide phosphorylase.
J Bacteriol. 1996 Jul;178(14):4281-8 PMID: 8763958
Mutational analysis of the Escherichia coli spoT gene identifies distinct but overlapping regions involved in ppGpp synthesis and degradation.
Mol Microbiol. 1996 Mar;19(6):1373-84 PMID: 8730877
Increase of methicillin resistance in Staphylococcus aureus caused by deletion of a gene whose product is homologous to lytic enzymes.
J Bacteriol. 1997 Oct;179(20):6294-301 PMID: 9335275
Cloning and characterization of a relA/spoT homologue from Bacillus subtilis.
Mol Microbiol. 1997 Oct;26(1):65-79 PMID: 9383190
The role of the Corynebacterium glutamicum rel gene in (p)ppGpp metabolism.
Microbiology. 1998 Jul;144 ( Pt 7):1853-62 PMID: 9695918
The relA/spoT-homologous gene in Streptomyces coelicolor encodes both ribosome-dependent (p)ppGpp-synthesizing and -degrading activities.
J Bacteriol. 1998 Aug;180(16):4123-32 PMID: 9696759
In vitro degradation of guanosine 5'-diphosphate, 3'-diphosphate.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5529-33 PMID: 414222

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.182.17.4995-4997.2000

dan_r_gentry@sbphrd.com

Published

2000-09-00

Pages

4995-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC111382

Subset

External Links

PubMed source DOI: 10.1128/JB.182.17.4995-4997.2000 PMC Full Text View journal details

Analysis Services

The rel gene is essential for in vitro growth of Staphylococcus aureus.

Contact

WeChat Official Account