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PMID: 10938361 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Ethylene perception by the ERS1 protein in Arabidopsis.

Plant physiology ·Vol. 123 ·No. 4 ·2000-08-00 ·Pages 1449-58

Hall AE, Findell JL, Schaller GE, Sisler EC, Bleecker AB

Abstract

Ethylene perception in Arabidopsis is controlled by a family of five genes, including ETR1, ERS1 (ethylene response sensor 1), ERS2, ETR2, and EIN4. ERS1, the most highly conserved gene with ETR1, encodes a protein with 67% identity to ETR1. To clarify the role of ERS1 in ethylene sensing, we biochemically characterized the ERS1 protein by heterologous expression in yeast. ERS1, like ETR1, forms a membrane-associated, disulfide-linked dimer. In addition, yeast expressing the ERS1 protein contains ethylene-binding sites, indicating ERS1 is also an ethylene-binding protein. This finding supports previous genetic evidence that isoforms of ETR1 also function in plants as ethylene receptors. Further, we used the ethylene antagonist 1-methylcyclopropene (1-MCP) to characterize the ethylene-binding sites of ERS1 and ETR1. We found 1-MCP to be both a potent inhibitor of the ethylene-induced seedling triple response, as well as ethylene binding by yeast expressing ETR1 and ERS1. Yeast expressing ETR1 and ERS1 showed nearly identical sensitivity to 1-MCP, suggesting that the ethylene-binding sites of ETR1 and ERS1 have similar affinities for ethylene.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins Cyclopropanes/pharmacology Ethylenes/metabolism Glutathione Transferase/genetics,metabolism Plant Proteins/genetics,metabolism Receptors, Cell Surface/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Arabidopsis Proteins Cyclopropanes ERS protein, Arabidopsis Ethylenes Plant Proteins Receptors, Cell Surface Recombinant Fusion Proteins ethylene receptors, plant ethylene Glutathione Transferase 1-methylcyclopropene
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hall A E
Department of Botany, University of Wisconsin, Madison, Wisconsin 53706, USA.
Findell J L
Schaller G E
Sisler E C
Bleecker A B
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.123.4.1449
Published
2000-08-00
Pages
1449-58
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC59101
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.123.4.1449 PMC Full Text View journal details
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