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PMID: 10935022 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

Use of PRKO mice to study the role of progesterone in mammary gland development.

Journal of mammary gland biology and neoplasia ·Vol. 2 ·No. 4 ·1997-10-00 ·Pages 343-54

Humphreys RC, Lydon JP, O'Malley BW, Rosen JM

Abstract

To better understand the distinct physiological roles played by progesterone and estrogen receptors (PR and ER) as well as to study directly PR function in an in vivo context, a novel mutant mouse strain, the PR knockout (PRKO) mouse, was generated carrying a germline loss of function mutation at the PR locus. Mouse mammary gland development has been examined in PRKO mice using reciprocal transplantation experiments to investigate the effects of the stromal and epithelial PRs on ductal and lobuloalveolar development. The absence of PR in transplanted donor epithelium, but not in recipient stroma, prevented normal lobuloalveolar development in response to estrogen and progesterone treatment. Conversely, the presence of PR in the transplanted donor epithelium, but not in the recipient stroma, revealed that PR in the stroma may be necessary for ductal development. Stimulation of ductal development by the PR may, therefore, be mediated by an unknown secondary signaling molecule, possibly a growth factor. The continued stimulation of the stromal PR appears to be dependent on reciprocal signal(s) from the epithelium. Thus, the combination of gene knockout and reciprocal transplantation technologies has provided some new insights into the role of stromal-epithelial interactions and steroid hormones in mammary gland development.

MeSH Terms
Animals Female Mammary Glands, Animal/embryology,physiology Mice Mice, Knockout Progesterone/physiology Receptors, Progesterone/physiology
Chemicals
Receptors, Progesterone Progesterone
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Humphreys R C
National Institutes of Health, Developmental Biology Section, Bethesda, Maryland 208922, USA.
Lydon J P
O'Malley B W
Rosen J M
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Article Info
Journal
Journal of mammary gland biology and neoplasia
Abbr.
J Mammary Gland Biol Neoplasia
ISSN
1083-3021
DOI
10.1023/a:1026343212187
Published
1997-10-00
Pages
343-54
Language
English
Region
United States
NLM ID
9601804
Subset
IM
Grants
NCI NIH HHS · CA64255 · United States
External Links
PubMed source DOI: 10.1023/a:1026343212187 View journal details
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