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PMID: 10933068 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Intravital fluorescence videomicroscopy to study tumor angiogenesis and microcirculation.

Neoplasia (New York, N.Y.) ·Vol. 2 ·No. 1-2 ·2000-00-00 ·Pages 53-61

Vajkoczy P, Ullrich A, Menger MD

Abstract

Angiogenesis and microcirculation play a central role in growth and metastasis of human neoplasms, and, thus, represent a major target for novel treatment strategies. Mechanistic analysis of processes involved in tumor vascularization, however, requires sophisticated in vivo experimental models and techniques. Intravital microscopy allows direct assessment of tumor angiogenesis, microcirculation and overall perfusion. Its application to the study of tumor-induced neovascularization further provides information on molecular transport and delivery, intra- and extravascular cell-to-cell and cell-to-matrix interaction, as well as tumor oxygenation and metabolism. With the recent advances in the field of bioluminescence and fluorescent reporter genes, appropriate for in vivo imaging, the intravital fluorescent microscopic approach has to be considered a powerful tool to study microvascular, cellular and molecular mechanisms of tumor growth.

MeSH Terms
Animals Endothelium, Vascular/cytology Humans Leukocytes/cytology Microcirculation/pathology Microscopy, Fluorescence/methods Microscopy, Video/methods Neoplasms/blood supply,diagnosis Neovascularization, Pathologic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vajkoczy P
Department of Neurosurgery, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany. peter.vajkoczy@nch.ma.uni.heidelberg.de
Ullrich A
Menger M D
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Article Info
Journal
Neoplasia (New York, N.Y.)
Abbr.
Neoplasia
ISSN
1522-8002
DOI
10.1038/sj.neo.7900062
Email
peter.vajkoczy@nch.ma.uni.heidelberg.de
Published
2000-00-00
Pages
53-61
Language
English
Region
United States
NLM ID
100886622
PMCID
PMC1531866
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.neo.7900062 PMC Full Text
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