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PMID: 1093172 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Replicative intermediates of colicin E1 plasmid DNA in minicells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 3 ·1975-03-00 ·Pages 829-33

Oka A, Inselburg J

Abstract

Pulse-labeled colicin E1 plasmid (Col E1) DNA in minicells was examined to characterize replicating molecules. Replication of Col E1 DNA principally occurred in covalently closed molecules and involved the synthesis of variable length single-stranded DNA fragments that were dissociable from the template DNA and ultimately incorporated into the completely replicated molecules. RNA was found to be associated with some of these newly synthesized fragments. Replicating molecules containing different size displacement loops were observed by electron microscopy.

MeSH Terms
Centrifugation, Density Gradient Colicins/biosynthesis DNA Replication DNA, Bacterial/analysis,biosynthesis DNA, Circular/biosynthesis DNA, Single-Stranded/biosynthesis Escherichia coli/metabolism Extrachromosomal Inheritance Microscopy, Electron Nucleic Acid Hybridization
Chemicals
Colicins DNA, Bacterial DNA, Circular DNA, Single-Stranded
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Oka A
Inselburg J
References (20)
20 references, click to expand
  1. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
    J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
  2. Replicating SV40 molecules containing closed circular template DNA strands.
    Nat New Biol. 1971 Sep 15;233(37):72-5 PMID: 4330296
  3. RNA in nascent DNA from cultured human lymphocytes.
    Nat New Biol. 1973 Oct 24;245(147):234-7 PMID: 4518365
  4. RNA-linked nascent DNA fragments in Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1863-7 PMID: 4558661
  5. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691-5 PMID: 4560696
  6. Replication of colicin E1 plasmid DNA in cell extracts.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):802-6 PMID: 4595573
  7. Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1403-7 PMID: 4598305
  8. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260-4 PMID: 4601821
  9. Evidence for the attachment of RNA to pulse-labeled DNA in the slime mold, Physarum polycephalum.
    Biochem Biophys Res Commun. 1973 Mar 5;51(1):174-80 PMID: 4735511
  10. Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis.
    Biochemistry. 1968 Oct;7(10):3513-20 PMID: 4878700
  11. Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli.
    J Bacteriol. 1970 Jun;102(3):642-7 PMID: 4914069
  12. A novel closed-circular mitochondrial DNA with properties of a replicating intermediate.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2252-7 PMID: 5289384
  13. MINIATURE escherichia coli CELLS DEFICIENT IN DNA.
    Proc Natl Acad Sci U S A. 1967 Feb;57(2):321-6 PMID: 16591472
  14. Electron microscopic studies of replicating and catenated colicin factor E1 DNA isolated from minicells (DNA replication).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):89-92 PMID: 4550513
  15. Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2518-22 PMID: 4560690
  16. Colicin factor DNA: a single non-homologous region in Col E2-E3 heteroduplex molecules.
    Nat New Biol. 1973 Feb 21;241(112):234-7 PMID: 4573128
  17. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256-9 PMID: 4601583
  18. RNA bound to nascent DNA in Ehrlich ascites tumor cells.
    Biochem Biophys Res Commun. 1972 Nov 1;49(3):827-34 PMID: 4674116
  19. Replicating DNA: structure of colicin factor E1.
    Science. 1970 Aug 7;169(3945):590-2 PMID: 4913300
  20. Isolation of catenated and replicating DNA molecules of colicin factor E1 from minicells.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2839-42 PMID: 4941988
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.3.829
Published
1975-03-00
Pages
829-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432413
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.3.829 PMC Full Text View journal details
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