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PMID: 1093139 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Photo-incuced cross-linkage of gene-5 protein and bacteriophage fd DNA+.

Nucleic acids research ·Vol. 2 ·No. 3 ·1975-03-00 ·Pages 361-71

Anderson E, Nakashima Y, Konigsberg W

Abstract

The gene 5 protein, coded for by the bacteriophage fd, forms a complex with single stranded fd-DNA such that one gene 5 protein monomer interacts with four bases. Exposure of this complex to ultraviolet light results in the formation of covalent bonds between 25-30% of the gene 5 protein monomers which are bound to the DNA. In contrast, when the intact fd virion, which is a complex of coat protein and DNA, was exposed to ultraviolet irradiation, no detectable protein DNA cross-links were found.

MeSH Terms
Bacterial Proteins/radiation effects Binding Sites Centrifugation, Density Gradient Coliphages DNA Viruses DNA, Viral/radiation effects Deoxyribonucleases Electrophoresis, Polyacrylamide Gel Escherichia coli Genes Pancreas/enzymology Protein Binding Radiation Effects Ultraviolet Rays
Chemicals
Bacterial Proteins DNA, Viral Deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Anderson E
Nakashima Y
Konigsberg W
References (24)
24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/2.3.361
Published
1975-03-00
Pages
361-71
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342842
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/2.3.361 PMC Full Text View journal details
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