Lipid synthesis in stringent Escherichia coli: an artifact in acetate labeling of phospholipids during a shiftdown in growth rate.

Journal of bacteriology ·Vol. 121 ·No. 1 ·1975-01-00 ·Pages 396-9

Nunn WD, Meade LA, Tropp BE

Abstract

The use of (14C)acetate to label the phospholipids of stringent Escherichia coli, after a decrease in agitation, leads to artifacts resulting from a decrease in the specific activity of the acetyl coenzyme A pool.

MeSH Terms

Acetates/metabolism Acetyl Coenzyme A/metabolism Arginine/metabolism Bacterial Proteins/biosynthesis Escherichia coli/growth & development,metabolism Genes Glucose/metabolism Lipids/biosynthesis Lysine/metabolism Phosphates/metabolism Phospholipids/biosynthesis Phosphorus Radioisotopes RNA, Bacterial/biosynthesis Tritium Uracil/metabolism

Chemicals

Acetates Bacterial Proteins Lipids Phosphates Phospholipids Phosphorus Radioisotopes RNA, Bacterial Tritium Uracil Acetyl Coenzyme A Arginine Glucose Lysine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Nunn W D

Meade L A

Tropp B E

References (12)

12 references, click to expand

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Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant.
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Stringent control of fatty acid synthesis in Escherichia coli. Possible regulation of acetyl coenzyme A carboxylase by ppGpp.
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Unsaturated fatty acid synthesis is not required for induction of lactose transport in Escherichia coli.
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rel Gene control of lipid synthesis in Escherichia coli. Evidence for eliminating fatty acid synthesis as the sole regulatory site.
J Biol Chem. 1974 Jun 25;249(12):3994-6 PMID: 4599645
Effects of phenethyl alcohol on phospholipid metabolism in Escherichia coli.
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Stringent and relaxed control of phospholipid metabolism in Escherichia coli.
J Biol Chem. 1972 Oct 25;247(20):6651-8 PMID: 4561941
RC gene control in Escherichia coli is not restricted to RNA synthesis.
Biochim Biophys Acta. 1970 Jan 21;199(1):256-64 PMID: 4905133
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.121.1.396-399.1975

Published

1975-01-00

Pages

396-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285656

Subset

External Links

PubMed source DOI: 10.1128/jb.121.1.396-399.1975 PMC Full Text View journal details

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Lipid synthesis in stringent Escherichia coli: an artifact in acetate labeling of phospholipids during a shiftdown in growth rate.

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