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PMID: 10899319 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of rat casein kinase 1epsilon.

FEBS letters ·Vol. 477 ·No. 1-2 ·2000-07-14 ·Pages 106-12

Takano A, Shimizu K, Kani S, Buijs RM, Okada M, Nagai K

Abstract

Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1epsilon (CK1epsilon) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1epsilon and characterization of the protein products. The rCK1epsilon is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells.

MeSH Terms

Alternative Splicing/genetics Amino Acid Sequence Animals Biological Clocks Biological Transport COS Cells Casein Kinases Cell Cycle Proteins Cell Nucleus/metabolism Circadian Rhythm Cloning, Molecular Gene Expression Profiling Isoenzymes/chemistry,genetics,metabolism Male Molecular Sequence Data Mutation/genetics Nuclear Proteins/metabolism Period Circadian Proteins Phosphorylation Protein Binding Protein Kinases/chemistry,genetics,metabolism RNA, Messenger/analysis,genetics Rats Rats, Wistar Recombinant Fusion Proteins/chemistry,genetics,metabolism Sequence Alignment Suprachiasmatic Nucleus/metabolism Transcription Factors

Chemicals

Cell Cycle Proteins Isoenzymes Nuclear Proteins PER1 protein, human PER2 protein, human Per1 protein, mouse Per1 protein, rat Per2 protein, mouse Per2 protein, rat Period Circadian Proteins RNA, Messenger Recombinant Fusion Proteins Transcription Factors Protein Kinases Casein Kinases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Takano A

Division of Protein Metabolism, Institute for Protein Research, Osaka University, 3-2 Yamado-Oka, Suita, Osaka, Japan. atsuko@protein.osaka-u.ac.jp

Shimizu K

Kani S

Buijs R M

Okada M

Nagai K

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/s0014-5793(00)01755-5

atsuko@protein.osaka-u.ac.jp

Published

2000-07-14

Pages

106-12

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/s0014-5793(00)01755-5 View journal details

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Cloning and characterization of rat casein kinase 1epsilon.

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