Home LiteratureArticle Details
PMID: 10890888 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription coupled repair of 8-oxoguanine in murine cells: the ogg1 protein is required for repair in nontranscribed sequences but not in transcribed sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 15 ·2000-07-18 ·Pages 8397-402

Le Page F, Klungland A, Barnes DE, Sarasin A, Boiteux S

Abstract

To assess the role of the Ogg1 DNA glycosylase in the transcription-coupled repair (TCR) of the mutagenic lesion, 7, 8-dihydro-8oxoguanine (8-OxoG), we have investigated the removal of this lesion in wild-type and ogg1(-/-) null mouse embryo fibroblast (MEF) cell lines. We used nonreplicating plasmids containing a single 8-OxoG.C base pair in a different assay that allowed us to study the removal of 8-OxoG located in a transcribed sequence (TS) or in a nontranscribed sequence (NTS). The results show that the removal of 8-OxoG in a wild-type MEF cell line is faster in the TS than in the NTS, indicating TCR of 8-OxoG in murine cells. In the homozygous ogg1(-/-) MEF cell line, 8-OxoG was not removed from the NTS whereas there was still efficient 8-OxoG repair in the TS. Expression of the mouse Ogg1 protein in the homozygous ogg1(-/-) cell line restored the ability to remove 8-OxoG in the NTS. Therefore, we have demonstrated that Ogg1 is essential for the repair of 8-OxoG in the NTS but is not required in the TS. These results indicate the existence of an Ogg1-independent pathway for the TCR of 8-OxoG in vivo.

MeSH Terms
Animals Cell Line, Transformed DNA Repair DNA-Formamidopyrimidine Glycosylase Guanine/analogs & derivatives Mice N-Glycosyl Hydrolases/genetics,metabolism Plasmids Transcription, Genetic
Chemicals
8-hydroxyguanine Guanine N-Glycosyl Hydrolases DNA-Formamidopyrimidine Glycosylase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Le Page F
Laboratoire de Radiobiologie de l'ADN, UMR217, Commissariat à l'Energie Atomique-Centre National de la Recherche Scientifique, BP6, 92265-Fontenay aux Roses, France.
Klungland A
Barnes D E
Sarasin A
Boiteux S
References (46)
46 references, click to expand
  1. Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage.
    Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13300-5 PMID: 10557315
  2. MSH2 and MSH6 are required for removal of adenine misincorporated opposite 8-oxo-guanine in S. cerevisiae.
    Mol Cell. 1999 Sep;4(3):439-44 PMID: 10518225
  3. DNA glycosylases in the base excision repair of DNA.
    Biochem J. 1997 Jul 1;325 ( Pt 1):1-16 PMID: 9224623
  4. Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.
    Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4156-61 PMID: 10725358
  5. Transcription-coupled repair of 8-oxoguanine: requirement for XPG, TFIIH, and CSB and implications for Cockayne syndrome.
    Cell. 2000 Apr 14;101(2):159-71 PMID: 10786832
  6. SV40-based shuttle viruses.
    Mutat Res. 1989 Mar-May;220(2-3):101-6 PMID: 2538733
  7. Homogeneous Escherichia coli FPG protein. A DNA glycosylase which excises imidazole ring-opened purines and nicks DNA at apurinic/apyrimidinic sites.
    J Biol Chem. 1990 Mar 5;265(7):3916-22 PMID: 1689309
  8. Chemical determination of free radical-induced damage to DNA.
    Free Radic Biol Med. 1991;10(3-4):225-42 PMID: 1650738
  9. The GO system protects organisms from the mutagenic effect of the spontaneous lesion 8-hydroxyguanine (7,8-dihydro-8-oxoguanine).
    J Bacteriol. 1992 Oct;174(20):6321-5 PMID: 1328155
  10. Description of a new amplifiable shuttle vector for mutagenesis studies in human cells: application to N-methyl-N'-nitro-N-nitrosoguanidine-induced mutation spectrum.
    Mutat Res. 1992 Oct;272(2):101-10 PMID: 1383742
  11. Mutagenesis by 8-oxoguanine: an enemy within.
    Trends Genet. 1993 Jul;9(7):246-9 PMID: 8379000
  12. Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis.
    J Biol Chem. 1993 Nov 5;268(31):23524-30 PMID: 8226881
  13. Preferential repair of ionizing radiation-induced damage in the transcribed strand of an active human gene is defective in Cockayne syndrome.
    Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10499-503 PMID: 8248136
  14. Reactive oxygen species in tumorigenesis.
    Cancer Res. 1994 Apr 1;54(7 Suppl):1890s-1894s PMID: 8137306
  15. Functional cooperation of MutT, MutM and MutY proteins in preventing mutations caused by spontaneous oxidation of guanine nucleotide in Escherichia coli.
    Mutat Res. 1995 May;336(3):257-67 PMID: 7739614
  16. DNA damage and cancer: measurement and mechanism.
    Cancer Lett. 1995 Jun 29;93(1):113-20 PMID: 7600538
  17. Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine.
    Proc Natl Acad Sci U S A. 1996 May 28;93(11):5197-202 PMID: 8643552
  18. Cloning and sequencing a human homolog (hMYH) of the Escherichia coli mutY gene whose function is required for the repair of oxidative DNA damage.
    J Bacteriol. 1996 Jul;178(13):3885-92 PMID: 8682794
  19. Cloning of a yeast 8-oxoguanine DNA glycosylase reveals the existence of a base-excision DNA-repair protein superfamily.
    Curr Biol. 1996 Aug 1;6(8):968-80 PMID: 8805338
  20. Defective transcription-coupled repair of oxidative base damage in Cockayne syndrome patients from XP group G.
    Science. 1997 Feb 14;275(5302):990-3 PMID: 9020084
  21. Inactivation of OGG1 increases the incidence of G . C-->T . A transversions in Saccharomyces cerevisiae: evidence for endogenous oxidative damage to DNA in eukaryotic cells.
    Mol Gen Genet. 1997 Mar 26;254(2):171-8 PMID: 9108279
  22. Cloning and characterization of mammalian 8-hydroxyguanine-specific DNA glycosylase/apurinic, apyrimidinic lyase, a functional mutM homologue.
    Cancer Res. 1997 Jun 1;57(11):2151-6 PMID: 9187114
  23. Cloning of a human homolog of the yeast OGG1 gene that is involved in the repair of oxidative DNA damage.
    Oncogene. 1997 Jun 12;14(23):2857-61 PMID: 9190902
  24. A mammalian DNA repair enzyme that excises oxidatively damaged guanines maps to a locus frequently lost in lung cancer.
    Curr Biol. 1997 Jun 1;7(6):397-407 PMID: 9197244
  25. Oxidative damage to DNA: formation, measurement, and biological significance.
    Rev Physiol Biochem Pharmacol. 1997;131:1-87 PMID: 9204689
  26. Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase.
    Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7429-34 PMID: 9207108
  27. Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8010-5 PMID: 9223305
  28. Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase.
    Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8016-20 PMID: 9223306
  29. In vitro repair of oxidative DNA damage by human nucleotide excision repair system: possible explanation for neurodegeneration in xeroderma pigmentosum patients.
    Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9463-8 PMID: 9256505
  30. Oxidative decay of DNA.
    J Biol Chem. 1997 Aug 8;272(32):19633-6 PMID: 9289489
  31. Differential involvement of the human mismatch repair proteins, hMLH1 and hMSH2, in transcription-coupled repair.
    Cancer Res. 1997 Sep 1;57(17):3784-91 PMID: 9288788
  32. Antisense insulin-like growth factor I transferred into a rat hepatoma cell line inhibits tumorigenesis by modulating major histocompatibility complex I cell surface expression.
    Cancer Gene Ther. 1997 Sep-Oct;4(5):276-85 PMID: 9345599
  33. Opposite base-dependent reactions of a human base excision repair enzyme on DNA containing 7,8-dihydro-8-oxoguanine and abasic sites.
    EMBO J. 1997 Oct 15;16(20):6314-22 PMID: 9321410
  34. The consequence of translesional replication of unique UV-induced photoproducts.
    Biol Chem. 1997 Nov;378(11):1287-92 PMID: 9426188
  35. Genomic structure and chromosomal localization of the mouse Ogg1 gene that is involved in the repair of 8-hydroxyguanine in DNA damage.
    Mamm Genome. 1998 Jan;9(1):32-7 PMID: 9434942
  36. Repair and mutagenic potency of 8-oxoG:A and 8-oxoG:C base pairs in mammalian cells.
    Nucleic Acids Res. 1998 Mar 1;26(5):1276-81 PMID: 9469837
  37. Mitochondrial targeting of human DNA glycosylases for repair of oxidative DNA damage.
    Nucleic Acids Res. 1998 Jun 15;26(12):2917-22 PMID: 9611236
  38. BRCA1 required for transcription-coupled repair of oxidative DNA damage.
    Science. 1998 Aug 14;281(5379):1009-12 PMID: 9703501
  39. The presence of two distinct 8-oxoguanine repair enzymes in human cells: their potential complementary roles in preventing mutation.
    Nucleic Acids Res. 1998 Nov 15;26(22):5116-22 PMID: 9801308
  40. Base excision repair of oxidative DNA damage activated by XPG protein.
    Mol Cell. 1999 Jan;3(1):33-42 PMID: 10024877
  41. Spontaneous mutation, oxidative DNA damage, and the roles of base and nucleotide excision repair in the yeast Saccharomyces cerevisiae.
    Yeast. 1999 Feb;15(3):205-18 PMID: 10077187
  42. Excision repair of 8-hydroxyguanine in mammalian cells: the mouse Ogg1 protein as a model.
    Free Radic Res. 1998 Dec;29(6):487-97 PMID: 10098454
  43. Base excision repair of 8-hydroxyguanine protects DNA from endogenous oxidative stress.
    Biochimie. 1999 Jan-Feb;81(1-2):59-67 PMID: 10214911
  44. Expression and differential intracellular localization of two major forms of human 8-oxoguanine DNA glycosylase encoded by alternatively spliced OGG1 mRNAs.
    Mol Biol Cell. 1999 May;10(5):1637-52 PMID: 10233168
  45. Overexpression of Ogg1 in mammalian cells: effects on induced and spontaneous oxidative DNA damage and mutagenesis.
    Carcinogenesis. 1999 Sep;20(9):1863-8 PMID: 10469635
  46. Quality control by DNA repair.
    Science. 1999 Dec 3;286(5446):1897-905 PMID: 10583946
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.140137297
Published
2000-07-18
Pages
8397-402
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC26959
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.140137297 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2