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PMID: 10877217 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Altered mucin core peptide expression in acute and chronic cholecystitis.

Digestive diseases and sciences ·Vol. 45 ·No. 6 ·2000-06-00 ·Pages 1061-71

Ho SB, Shekels LL, Toribara NW, Gipson IK, Kim YS, Purdum PP, Cherwitz DL

Abstract

Human mucin genes include membrane-bound mucins (MUC1, MUC3, MUC4) and secretory mucins (MUC2, MUC5AC, MUC5B, MUC6). Our aim was to determine mucin gene expression in human gallbladder cell lines, normal gallbladder from liver donors (N = 7) and surgical specimens with mild chronic cholecystitis (N = 29), chronic cholecystitis (N = 48), and acute and chronic cholecystitis (N = 27). MUC1 mRNA was ubiquitous; however, only rare MUC1 immunoreactivity was detected. MUC3, MUC5AC, MUC5B, and MUC6 mRNA were present in all gallbladder specimens and cell lines examined. Prominent MUC3, MUC5AC, MUC5B, and MUC6 immunoreactivity was present in 86-100% of normal gallbladders. The frequency of MUC5AC reactivity was decreased in specimens with acute cholecystitis (P < 0.05). In contrast, MUC2-reactivity was absent in normal gallbladder and present in 53.8% of acute cholecystitis specimens (P < 0.05). Surface epithelium is characterized by MUC3, MUC5AC, and MUC5B, whereas deeper mucosal folds display MUC5B and MUC6 immunoreactivity. Gallbladder epithelium demonstrates a unique and diverse pattern of mucin core proteins that becomes altered with increasing degrees of inflammation.

MeSH Terms
Acute Disease Cell Line Cholecystitis/metabolism Chronic Disease Digestive System Physiological Phenomena Epithelial Cells/physiology Epitopes/metabolism Gallbladder/physiology Gene Expression Humans Immunohistochemistry Mucins/genetics,immunology,metabolism Peptide Fragments/metabolism RNA, Messenger/metabolism Reference Values
Chemicals
Epitopes Mucins Peptide Fragments RNA, Messenger
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ho S B
Department of Medicine, Veterans Affairs Medical Center and University of Minnesota, Minneapolis 55417, USA.
Shekels L L
Toribara N W
Gipson I K
Kim Y S
Purdum P P
Cherwitz D L
References (57)
57 references, click to expand
  1. Mucin-producing cells and endocrine cells of gallbladder epithelium in patients with uncomplicated cholelithiasis.
    Hepatogastroenterology. 1991 Apr;38(2):149-53 PMID: 1855773
  2. Molecular cloning of a major human gall bladder mucin: complete C-terminal sequence and genomic organization of MUC5B.
    Biochem J. 1997 May 15;324 ( Pt 1):295-303 PMID: 9164870
  3. Human gallbladder mucin binds biliary lipids and promotes cholesterol crystal nucleation in model bile.
    J Lipid Res. 1987 Sep;28(9):1088-97 PMID: 3655561
  4. Isolation of mucin from human hepatic bile and its induced effects on precipitation of cholesterol and calcium carbonate in vitro.
    Dig Dis Sci. 1993 May;38(5):909-15 PMID: 8482190
  5. Heterogeneity of mucin gene expression in normal and neoplastic tissues.
    Cancer Res. 1993 Feb 1;53(3):641-51 PMID: 7678777
  6. Effect of mucous glycoprotein on nucleation time of human bile.
    Gastroenterology. 1985 Sep;89(3):648-58 PMID: 4018505
  7. Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5).
    J Biol Chem. 1994 Apr 29;269(17):12932-9 PMID: 7513696
  8. Purification and characterization of sialyl-Le(a)-carrying mucins of human bile; evidence for the presence of MUC1 and MUC3 apoproteins.
    J Biol Chem. 1994 May 20;269(20):14430-7 PMID: 7848403
  9. Human gastric mucin. Identification of a unique species by expression cloning.
    J Biol Chem. 1993 Mar 15;268(8):5879-85 PMID: 7680650
  10. Northern blot normalization with a 28S rRNA oligonucleotide probe.
    Nucleic Acids Res. 1989 Sep 12;17(17):7115 PMID: 2674908
  11. Cloning and characterization of mouse intestinal MUC3 mucin: 3' sequence contains epidermal-growth-factor-like domains.
    Biochem J. 1998 Mar 15;330 ( Pt 3):1301-8 PMID: 9494100
  12. Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7).
    J Biol Chem. 1993 Sep 25;268(27):20563-9 PMID: 7690757
  13. Bovine gallbladder mucin promotes cholesterol crystal nucleation from cholesterol-transporting vesicles in supersaturated model bile.
    J Lipid Res. 1989 Apr;30(4):491-8 PMID: 2754332
  14. MUC5B is the prominent mucin in human gallbladder and is also expressed in a subset of colonic goblet cells.
    Am J Physiol. 1998 May;274(5 Pt 1):G871-8 PMID: 9612268
  15. Gallbladder mucin, arachidonic acid, and bile lipids in patients who develop gallstones during weight reduction.
    Gastroenterology. 1993 Oct;105(4):1200-8 PMID: 8405867
  16. Evidence for different human tracheobronchial mucin peptides deduced from nucleotide cDNA sequences.
    Am J Respir Cell Mol Biol. 1991 Aug;5(2):178-85 PMID: 1892649
  17. A novel human airway mucin cDNA encodes a protein with unique tandem-repeat organization.
    Biochem J. 1994 Jun 1;300 ( Pt 2):295-8 PMID: 8002930
  18. Hydrophobic binding properties of bovine gallbladder mucin.
    J Biol Chem. 1984 Oct 10;259(19):12170-7 PMID: 6480603
  19. Intestinal metaplasia of the gallbladder: a morphologic and immunocytochemical study.
    Hum Pathol. 1986 Jun;17(6):614-20 PMID: 2872152
  20. Differential mucin gene expression in human pancreatic and colon cancer cells.
    Biochem J. 1991 Jun 15;276 ( Pt 3):599-605 PMID: 2064602
  21. Human mucin gene MUC4: organization of its 5'-region and polymorphism of its central tandem repeat array.
    Biochem J. 1998 Jun 15;332 ( Pt 3):739-48 PMID: 9620877
  22. The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region.
    J Biol Chem. 1992 Oct 25;267(30):21375-83 PMID: 1400449
  23. Altered expression of MUC2, MUC4, and MUC5 mucin genes in pancreas tissues and cancer cell lines.
    Gastroenterology. 1994 Apr;106(4):1054-61 PMID: 8143972
  24. Mucin gene expression in normal, preneoplastic, and neoplastic human gastric epithelium.
    Cancer Res. 1995 Jun 15;55(12):2681-90 PMID: 7780985
  25. Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs.
    Biochem Biophys Res Commun. 1991 Mar 15;175(2):414-22 PMID: 1673336
  26. Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin.
    J Biol Chem. 1990 Sep 5;265(25):15286-93 PMID: 1697589
  27. Degenerate 87-base-pair tandem repeats create hydrophilic/hydrophobic alternating domains in human mucin peptides mapped to 11p15.
    Biochem J. 1993 Jul 15;293 ( Pt 2):329-37 PMID: 7916618
  28. Human mucin gene MUC5B, the 10.7-kb large central exon encodes various alternate subdomains resulting in a super-repeat. Structural evidence for a 11p15.5 gene family.
    J Biol Chem. 1997 Feb 7;272(6):3168-78 PMID: 9013550
  29. Genomic organization of the 3' region of the human mucin gene MUC5B.
    J Biol Chem. 1997 Jul 4;272(27):16873-83 PMID: 9201995
  30. Alternating laminated array of two types of mucin in the human gastric surface mucous layer.
    Histochem J. 1992 Feb;24(2):86-92 PMID: 1374372
  31. Aspirin prevention of cholesterol gallstone formation in prairie dogs.
    Science. 1981 Mar 27;211(4489):1429-31 PMID: 7466399
  32. Cloning of partial cDNA encoding differentiation and tumor-associated mucin glycoproteins expressed by human mammary epithelium.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6060-4 PMID: 2888110
  33. Complexity of MAM-6, an epithelial sialomucin associated with carcinomas.
    Cancer Res. 1989 Feb 15;49(4):786-93 PMID: 2643463
  34. Mucus: the front line of intestinal mucosal defense.
    Ann N Y Acad Sci. 1992;664:190-201 PMID: 1456650
  35. Differential apomucin expression in normal and neoplastic human gastrointestinal tissues.
    Gastroenterology. 1994 Jul;107(1):160-72 PMID: 8020658
  36. Aberrant expression of MUC5AC and MUC6 gastric mucin genes in colorectal polyps.
    Int J Cancer. 1999 Jan 18;80(2):210-8 PMID: 9935202
  37. Improved hybridization assays employing tailed oligonucleotide probes: a direct comparison with 5'-end-labeled oligonucleotide probes and nick-translated plasmid probes.
    Anal Biochem. 1985 Dec;151(2):211-24 PMID: 3913326
  38. Molecular cloning of human intestinal mucin cDNAs. Sequence analysis and evidence for genetic polymorphism.
    J Biol Chem. 1989 Apr 15;264(11):6480-7 PMID: 2703501
  39. The human tumour-associated epithelial mucins are coded by an expressed hypervariable gene locus PUM.
    Nature. 1987 Jul 2-8;328(6125):82-4 PMID: 3600778
  40. Localization of mucin (MUC2 and MUC3) messenger RNA and peptide expression in human normal intestine and colon cancer.
    Gastroenterology. 1994 Jul;107(1):28-36 PMID: 8020672
  41. Nature and composition of biliary sludge.
    Gastroenterology. 1986 Mar;90(3):677-86 PMID: 3943697
  42. Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin.
    Biochem J. 1999 Mar 1;338 ( Pt 2):325-33 PMID: 10024507
  43. MUC3 human intestinal mucin. Analysis of gene structure, the carboxyl terminus, and a novel upstream repetitive region.
    J Biol Chem. 1997 Oct 17;272(42):26678-86 PMID: 9334251
  44. UW-preservation of cultured human gallbladder epithelial cells: phenotypic alterations and differential mucin gene expression in the presence of bile.
    Hepatology. 1995 Jan;21(1):223-31 PMID: 7806158
  45. Pathogenesis of biliary sludge.
    Hepatology. 1990 Sep;12(3 Pt 2):200S-203S; discussion 203S-205S PMID: 2210650
  46. Molecular cloning of cDNAs derived from a novel human intestinal mucin gene.
    Biochem Biophys Res Commun. 1990 Aug 31;171(1):407-15 PMID: 2393399
  47. Expression of human mucin genes in respiratory, digestive, and reproductive tracts ascertained by in situ hybridization.
    J Histochem Cytochem. 1993 Oct;41(10):1479-85 PMID: 8245407
  48. Characterization of the human mucin gene MUC5AC: a consensus cysteine-rich domain for 11p15 mucin genes?
    Biochem J. 1995 Jan 1;305 ( Pt 1):211-9 PMID: 7826332
  49. Expression cloning of gastric mucin complementary DNA and localization of mucin gene expression.
    Gastroenterology. 1995 Sep;109(3):735-47 PMID: 7657101
  50. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  51. Mucin-type glycoproteins.
    Crit Rev Biochem Mol Biol. 1992;27(1-2):57-92 PMID: 1727693
  52. Cultured human gallbladder epithelia. Methods and partial characterization of a carcinoma-derived model.
    Lab Invest. 1993 Mar;68(3):345-53 PMID: 7680730
  53. Gallbladder mucin as a pronucleating agent for cholesterol monohydrate crystals in bile.
    Hepatology. 1990 Sep;12(3 Pt 2):183S-186S; discussion 186S-188S PMID: 2210647
  54. Characterization of bovine gallbladder mucin. Amino acid sequences of tryptic peptides from the glycosylated domain of the protein core.
    Gastroenterology. 1990 Nov;99(5):1493-501 PMID: 2210258
  55. Isolation and characterization of peptides from the protein core of bovine gallbladder mucin.
    Gastroenterology. 1990 Jun;98 (6):1633-41 PMID: 2186955
  56. Intestinal metaplasia-dysplasia-carcinoma sequence of the gallbladder.
    Acta Pathol Jpn. 1986 Jul;36(7):989-97 PMID: 3529813
  57. The carboxyl-terminal sequence of the human secretory mucin, MUC6. Analysis Of the primary amino acid sequence.
    J Biol Chem. 1997 Jun 27;272(26):16398-403 PMID: 9195947
Article Info
Journal
Digestive diseases and sciences
Abbr.
Dig Dis Sci
ISSN
0163-2116
DOI
10.1023/a:1005573213100
Published
2000-06-00
Pages
1061-71
Language
English
Region
United States
NLM ID
7902782
Subset
IM
Grants
NICHD NIH HHS · R01-HD33171 · United States
External Links
PubMed source DOI: 10.1023/a:1005573213100 View journal details
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