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PMID: 10872313 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Microtubule-associated protein MAP2 preferentially binds to a dA/dT sequence present in mouse satellite DNA.

The EMBO journal ·Vol. 2 ·No. 8 ·1983-00-00 ·Pages 1229-34

Avila J, Montejo de Garcini E, Wandosell F, Villasante A, Sogo JM, Villanueva N

Abstract

Microtubule-associated protein MAP2 binds to the Sau96.1 restriction monomer fragment of mouse satellite DNA. This fragment is also present in a lower proportion in bulk DNA. The digestion of MAP2-Sau96.1 fragment complex by DNase results in the protection of certain nucleotide sequences. The sequence poly(dA)4/poly(dT)4 is mainly protected against DNase digestion.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Binding Sites DNA, Satellite/metabolism Deoxyribonucleases, Type II Site-Specific Mice Microtubule-Associated Proteins/metabolism Molecular Sequence Data Polydeoxyribonucleotides/metabolism Swine
Chemicals
DNA, Satellite Microtubule-Associated Proteins Polydeoxyribonucleotides polydeoxyadenylic acid-polythymidylic acid Deoxyribonucleases, Type II Site-Specific GATC-specific type II deoxyribonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Avila J
Centro de Biologia Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Montejo de Garcini E
Wandosell F
Villasante A
Sogo J M
Villanueva N
References (26)
26 references, click to expand
  1. Studies on the binding of RNA polymerase to polynucleotides.
    J Mol Biol. 1966 Dec 28;22(2):199-209 PMID: 5972769
  2. Site-specific DNA condensation and pairing mediated by the int protein of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5837-41 PMID: 6310548
  3. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Electron-microscopic study of the spindle and chromosome movement in the yeast Saccharomyces cerevisiae.
    J Cell Sci. 1976 Nov;22(2):219-42 PMID: 794073
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Binding of microtubule proteins to DNA: specificity of the interaction.
    Eur J Biochem. 1978 May 16;86(2):473-9 PMID: 207527
  8. Preferential binding of hog brain microtubule-associated proteins to mouse satellite versus bulk DNA preparations.
    Nature. 1978 Jun 1;273(5661):403-5 PMID: 661954
  9. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
    Nucleic Acids Res. 1978 Sep;5(9):3157-70 PMID: 212715
  10. RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1979 Feb 5;127(4):411-36 PMID: 107317
  11. Evidence for transposition of dispersed repetitive DNA families in yeast.
    Cell. 1979 Apr;16(4):739-51 PMID: 378399
  12. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
  13. Effects of DNA on microtubule assembly.
    Eur J Biochem. 1980 Mar;105(1):7-16 PMID: 6154575
  14. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  15. Different tubulin polymers are produced by microtubule-associated proteins MAP2 and tau in the presence of guanosine 5'-(alpha, beta-methylene)triphosphate.
    J Biol Chem. 1980 Oct 10;255(19):8952-4 PMID: 6773956
  16. Isolation of a yeast centromere and construction of functional small circular chromosomes.
    Nature. 1980 Oct 9;287(5782):504-9 PMID: 6999364
  17. Local decondensation of chromatic at the surface of meiotic chromosomes during anaphase movement.
    Eur J Cell Biol. 1980 Dec;23(1):166-70 PMID: 7460963
  18. Nucleotide sequence of mouse satellite DNA.
    Nucleic Acids Res. 1981 Feb 11;9(3):683-96 PMID: 6261227
  19. Structure of the mammalian kinetochore.
    Chromosoma. 1981;82(2):153-70 PMID: 7227036
  20. Binding of microtubule protein to DNA and chromatin: possibility of simultaneous linkage of microtubule to nucleic and assembly of the microtubule structure.
    Nucleic Acids Res. 1981 Feb 25;9(4):895-908 PMID: 7232207
  21. Comparative measurement by radioimmunoassay of the brain microtubule-associated protein MAP2.
    Mol Cell Biochem. 1981 Jul;37(3):185-9 PMID: 7278861
  22. Interspersion of mouse satellite deoxyribonucleic acid sequences.
    Biochemistry. 1981 Jul 21;20(15):4393-8 PMID: 6269590
  23. Quantitation and characterization of the microtubule associated MAP2 in porcine tissues and its isolation from porcine (PK15) and human (HeLa) cell lines.
    Biochem Biophys Res Commun. 1982 Apr 29;105(4):1241-9 PMID: 7049162
  24. Nucleotide sequence comparisons and functional analysis of yeast centromere DNAs.
    Cell. 1982 May;29(1):235-44 PMID: 7049398
  25. Protein D1 preferentially binds A + T-rich DNA in vitro and is a component of Drosophila melanogaster nucleosomes containing A + T-rich satellite DNA.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7152-6 PMID: 6818540
  26. Partial sequence analysis of mouse satellite DNA evidence for short range periodicities.
    J Mol Biol. 1975 May 5;94(1):71-86 PMID: 806696
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1229-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555265
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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