Cleavage map of bacteriophage phiX174 RF DNA by restriction enzymes.

Nucleic acids research ·Vol. 3 ·No. 8 ·1976-08-00 ·Pages 1947-60

Baas PD, van Heusden GP, Vereijken JM, Weisbeek PJ, Jansz HS

Abstract

phiX RF DNA was cleaved by restriction enzymes from Haemophilus influenzae Rf (Hinf I) and Haemophilus haemolyticus (Hha. I). Twenty one fragments of approximately 25 to 730 base pairs were produced by Hinf I and seventeen fragments of approximately 40 to 1560 base pairs by Hha I. The order of these fragments has been established by digestion on Haemophilus awgyptius (Hae III) and Arthrobacter luteus (Alu I) endonuclease fragments of phiX RF with Hinf I and Hha1. By this method of reciprocal digestion a detailed cleavage map of phiX RF DNA was constructed, which includes also the previously determined Hind II, Hae III and Alu I cleavage maps of phiX 174 RF DNA (1, 2). Moreover, 28 conditional lethal mutants of bacteriophage phiX174 were placed in this map using the genetic fragment assay (3).

MeSH Terms

Coliphages/analysis DNA Restriction Enzymes DNA, Circular DNA, Viral Endonucleases Genes, Lethal Haemophilus/enzymology Haemophilus influenzae/enzymology Molecular Weight Mutation Oligodeoxyribonucleotides/analysis

Chemicals

DNA, Circular DNA, Viral Oligodeoxyribonucleotides Endonucleases DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Baas P D

van Heusden G P

Vereijken J M

Weisbeek P J

Jansz H S

References (25)

25 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.8.1947

Published

1976-08-00

Pages

1947-60

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343051

Subset

External Links

PubMed source DOI: 10.1093/nar/3.8.1947 PMC Full Text View journal details

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Cleavage map of bacteriophage phiX174 RF DNA by restriction enzymes.

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