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PMID: 10852897 Published · ppublish English Journal Article

First evidence for existence of an uphill electron transfer through the bc(1) and NADH-Q oxidoreductase complexes of the acidophilic obligate chemolithotrophic ferrous ion-oxidizing bacterium Thiobacillus ferrooxidans.

Journal of bacteriology ·Vol. 182 ·No. 12 ·2000-06-00 ·Pages 3602-6

Elbehti A, Brasseur G, Lemesle-Meunier D

Abstract

The energy-dependent electron transfer pathway involved in the reduction of pyridine nucleotides which is required for CO(2) fixation to occur in the acidophilic chemolithotrophic organism Thiobacillus ferrooxidans was investigated using ferrocytochrome c as the electron donor. The experimental results show that this uphill pathway involves a bc(1) and an NADH-Q oxidoreductase complex functioning in reverse, using an electrochemical proton gradient generated by ATP hydrolysis. Based on these results, a model is presented to explain the balance of the reducing equivalent from ferrocytochrome c between the exergonic and endergonic electron transfer pathways.

MeSH Terms
Biological Transport, Active Cytochrome c Group/metabolism Electron Transport Electron Transport Complex I Electron Transport Complex III/metabolism Energy Metabolism Ferrous Compounds/metabolism NAD/metabolism NADH, NADPH Oxidoreductases/metabolism Oxidation-Reduction Oxygen/metabolism Thiobacillus/enzymology,metabolism
Chemicals
Cytochrome c Group Ferrous Compounds NAD NADH, NADPH Oxidoreductases Electron Transport Complex I Electron Transport Complex III Oxygen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elbehti A
Bioénergétique et Ingénierie des Proteines, CNRS, Institut de Biologie Structurale et Microbiologie, 13402 Marseille, cedex 20, France.
Brasseur G
Lemesle-Meunier D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.12.3602-3606.2000
Published
2000-06-00
Pages
3602-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC101977
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.182.12.3602-3606.2000 PMC Full Text View journal details
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