Characterization of an ampicillin-resistant Haemophilus influenzae type B.

Antimicrobial agents and chemotherapy ·Vol. 9 ·No. 6 ·1976-06-00 ·Pages 889-92

Eickhoff TC, Ehret JM, Baines RD

Abstract

A 28-year-old female in Denver was found in early 1974 to have frontal sinusitis, osteomyelitis, and bacteremia due to Haemophilus influenzae, type B. The minimal inhibitory concentration of ampicillin for this organism was 100 mug/ml and the minimal bactericidal concentration was >100 mug/ml. It was inhibited by chloramphenicol at 0.4 mug/ml. Further studies demonstrated that ampicillin and methicillin were synergistic against this organism. It was shown to produce a diffusible beta-lactamase. Transferase of resistance from this organism to a susceptible Haemophilus parainfluenzae and a reciprocal transfer were accomplished. A test for transformation was negative as was a test for reversal of resistance by ethylenediaminetetraacetic acid.

MeSH Terms

Adult Ampicillin/pharmacology Drug Synergism Female Haemophilus Infections/microbiology Haemophilus influenzae/drug effects,enzymology Humans Microbial Sensitivity Tests Penicillin Resistance Penicillinase/metabolism Time Factors Transformation, Genetic

Chemicals

Ampicillin Penicillinase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Eickhoff T C

Ehret J M

Baines R D

References (15)

15 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.9.6.889

Published

1976-06-00

Pages

889-92

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC429645

Subset

External Links

PubMed source DOI: 10.1128/AAC.9.6.889 PMC Full Text View journal details

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Characterization of an ampicillin-resistant Haemophilus influenzae type B.

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