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PMID: 10846101 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of human immunodeficiency virus type 2 Vpx and invariant chain.

Journal of virology ·Vol. 74 ·No. 13 ·2000-07-00 ·Pages 6168-72

Pancio HA, Vander Heyden N, Kosuri K, Cresswell P, Ratner L

Abstract

Vpx is a virion-associated protein of human immunodeficiency virus type 2 (HIV-2) and simian immunodeficiency viruses. The yeast two-hybrid system was used to identify invariant chain (Ii) as a cellular protein that interacts with HIV-2 Vpx. Vpx-Ii interaction was confirmed in cell-free reactions using bacterially expressed glutathione S-transferase fusion proteins and by coimmunoprecipitation in transfected and infected cells. In chronically infected cells expressing Vpx, Ii levels were markedly decreased, presumably due to enhanced degradation. These findings suggest that Vpx may disrupt major histocompatibility complex class II antigen presentation.

MeSH Terms
Antigens, Differentiation, B-Lymphocyte/genetics,metabolism Binding Sites HIV-2/immunology,metabolism HeLa Cells Histocompatibility Antigens Class II/genetics,metabolism Humans Nuclear Proteins Saccharomyces cerevisiae Trans-Activators/genetics,metabolism Transfection Two-Hybrid System Techniques Viral Regulatory and Accessory Proteins/genetics,immunology,metabolism
Chemicals
Antigens, Differentiation, B-Lymphocyte Histocompatibility Antigens Class II MHC class II transactivator protein Nuclear Proteins Trans-Activators VPX protein, Human immunodeficiency virus 2 Viral Regulatory and Accessory Proteins invariant chain
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pancio H A
Department of Medicine, Pathology, and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Vander Heyden N
Kosuri K
Cresswell P
Ratner L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.74.13.6168-6172.2000
Published
2000-07-00
Pages
6168-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC112116
Subset
IM
Grants
NIAID NIH HHS · T32 AI007172 · United States
NIAID NIH HHS · AI07172 · United States
NIAID NIH HHS · AI34736 · United States
External Links
PubMed source DOI: 10.1128/jvi.74.13.6168-6172.2000 PMC Full Text View journal details
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