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PMID: 1083421 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Growth of B-lymphocytes clones in semisolid culture is mitogen dependent.

The Journal of experimental medicine ·Vol. 143 ·No. 5 ·1976-05-01 ·Pages 1265-70

Kincade PW, Ralph P, Moore MA

Abstract

A substance which was mitogenic for murine B lymphocytes in the presence of 2-mercaptoethanol was isolated from agar. Stimulating activity of this material was stable to proteolysis or protein denaturants but was destroyed by periodate treatment. Agar-derived mitogen stimulation was distinct from that obtained with dextran sulfate or lipopolysaccharide and may define different populations of B lymphocytes.

MeSH Terms
Agar Animals B-Lymphocytes Cell Division Clone Cells Mice Mice, Inbred CBA Mice, Nude Mitogens Polysaccharides/pharmacology
Chemicals
Mitogens Polysaccharides Agar
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kincade P W
Ralph P
Moore M A
References (14)
14 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.143.5.1265
Published
1976-05-01
Pages
1265-70
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190186
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.143.5.1265 PMC Full Text View journal details
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