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PMID: 10816550 Published · ppublish English Journal Article

Characterization of an anticryptococcal protein isolated from human serum.

Infection and immunity ·Vol. 68 ·No. 6 ·2000-06-00 ·Pages 3787-91

Sridhar S, Ahluwalia M, Brummer E, Stevens DA

Abstract

Human serum at low concentrations inhibits the growth of Cryptococcus neoformans in vitro. Fractionation of serum yielded a purified inhibitory protein with a molecular mass of approximately 81.8 kDa, a pI of approximately 6.2, and an amino acid sequence that matched that of human transferrin. The inhibitory activity and that of apotransferrin and 5% human serum were reversed by 10 microM freshly prepared FeCl(3).

MeSH Terms
Antifungal Agents/isolation & purification,pharmacology Apoproteins/isolation & purification,pharmacology Blood Proteins/isolation & purification,pharmacology Chlorides Cryptococcus neoformans/drug effects Ferric Compounds/pharmacology Humans Immunity, Innate Transferrin/isolation & purification,pharmacology
Chemicals
Antifungal Agents Apoproteins Blood Proteins Chlorides Ferric Compounds Transferrin apotransferrin ferric chloride
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sridhar S
Division of Infectious Diseases, Department of Medicine, Santa Clara Valley Medical Center, and California Institute for Medical Research, San Jose, California, USA.
Ahluwalia M
Brummer E
Stevens D A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.68.6.3787-3791.2000
Published
2000-06-00
Pages
3787-91
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97681
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.68.6.3787-3791.2000 PMC Full Text View journal details
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