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PMID: 10810150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Identification, purification, and molecular cloning of a putative plastidic glucose translocator.

The Plant cell ·Vol. 12 ·No. 5 ·2000-05-00 ·Pages 787-802

Weber A, Servaites JC, Geiger DR, Kofler H, Hille D, Gröner F, Hebbeker U, Flügge UI

Abstract

During photosynthesis, part of the fixed carbon is directed into the synthesis of transitory starch, which serves as an intermediate carbon storage facility in chloroplasts. This transitory starch is mobilized during the night. Increasing evidence indicates that the main route of starch breakdown proceeds by way of hydrolytic enzymes and results in glucose formation. This pathway requires a glucose translocator to mediate the export of glucose from the chloroplasts. We have reexamined the kinetic properties of the plastidic glucose translocator and, using a differential labeling procedure, have identified the glucose translocator as a component of the inner envelope membrane. Peptide sequence information derived from this protein was used to isolate cDNA clones encoding a putative plastidic glucose translocator from spinach, potato, tobacco, Arabidopsis, and maize. We also present the molecular characterization of a candidate for a hexose transporter of the plastid envelope membrane. This transporter, initially characterized more than 20 years ago, is closely related to the mammalian glucose transporter GLUT family and differs from all other plant hexose transporters that have been characterized to date.

MeSH Terms
Amino Acid Sequence Base Sequence Chloroplasts/metabolism Cloning, Molecular DNA Primers DNA, Complementary Intracellular Membranes/metabolism Molecular Sequence Data Monosaccharide Transport Proteins/antagonists & inhibitors,genetics,isolation & purification,metabolism Mutation Phenotype Substrate Specificity
Chemicals
DNA Primers DNA, Complementary Monosaccharide Transport Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Weber A
Universität zu Köln, Lehrstuhl Botanik II, Gyrhofstrasse 15, D-50931 Cologne, Germany. andr.weber@uni-koeln.de
Servaites J C
Geiger D R
Kofler H
Hille D
Gröner F
Hebbeker U
Flügge U I
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.12.5.787
Email
andr.weber@uni-koeln.de
Published
2000-05-00
Pages
787-802
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139927
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.12.5.787 PMC Full Text View journal details
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