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PMID: 10810092 Published · ppublish English Comparative Study Journal Article

A radiation hybrid map of the cat genome: implications for comparative mapping.

Genome research ·Vol. 10 ·No. 5 ·2000-05-00 ·Pages 691-702

Murphy WJ, Sun S, Chen Z, Yuhki N, Hirschmann D, Menotti-Raymond M, O'Brien SJ

Abstract

Ordered gene maps of mammalian species are becoming increasingly valued in assigning gene variants to function in human and animal models, as well as recapitulating the natural history of genome organization. To extend this power to the domestic cat, a radiation hybrid (RH) map of the cat was constructed integrating 424 Type I-coding genes with 176 microsatellite markers, providing coverage over all 20 feline chromosomes. Alignment of parallel RH maps of human and cat reveal 100 conserved segments ordered (CSOs) between the species, nearly three times the number observed with reciprocal chromosome painting analyses. The observed number is equivalent to theoretical predictions of the number of conserved segments to be found between cat and human, implying that 300-400 Type I gene markers is sufficient to reveal nearly all conserved segments for species that exhibit the most frequently observed "slow" rate of genome reorganization. The cat-human RH map comparisons provide a new genomic tool for comparative gene mapping in the cat and related Felidae, and provide confirmation that the cat genome organization is remarkably conserved compared with human. These data demonstrate that ordered RH-based gene maps provide the most precise assessment of comparing genomes, short of contig construction or full-sequence determination.

MeSH Terms
Animals Cats Chromosome Mapping/methods Chromosome Painting Genome Genome, Human Humans Hybrid Cells Microsatellite Repeats Molecular Sequence Data
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Murphy W J
Laboratory of Genomic Diversity, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201 USA. murphywi@mail.ncifcrf.gov
Sun S
Chen Z
Yuhki N
Hirschmann D
Menotti-Raymond M
O'Brien S J
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.10.5.691
Email
murphywi@mail.ncifcrf.gov
Published
2000-05-00
Pages
691-702
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC310870
Subset
IM
Databases
GENBANK
AZ081249, AZ081250, AZ081251, AZ081252, AZ081253, AZ081254, AZ081255, AZ081256, AZ081257, AZ081258, AZ081259, AZ081260, AZ081261, AZ081262, AZ081263, AZ081264, AZ081265, AZ081266, AZ081267, AZ081268, AZ081269, AZ081270, AZ081271, AZ081272, AZ081273, AZ081274, AZ081275, AZ081276, AZ081277, AZ081278
External Links
PubMed source DOI: 10.1101/gr.10.5.691 PMC Full Text View journal details
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