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PMID: 10809715 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Organization, structure, and variability of the rRNA operon of the Whipple's disease bacterium (Tropheryma whippelii).

Journal of bacteriology ·Vol. 182 ·No. 11 ·2000-06-00 ·Pages 3292-7

Maiwald M, von Herbay A, Lepp PW, Relman DA

Abstract

Whipple's disease is a systemic disorder associated with a cultivation-resistant, poorly characterized actinomycete, Tropheryma whippelii. We determined a nearly complete rRNA operon sequence of T. whippelii from specimens from 3 patients with Whipple's disease, as well as partial operon sequences from 43 patients. Variability was observed in the 16S-23S rRNA spacer sequences, leading to the description of five distinct sequence types. One specimen contained two spacer sequence types, raising the possibility of a double infection. Secondary structure models for the primary rRNA transcript and mature rRNAs revealed rare or unique features.

MeSH Terms
Actinomycetales/classification,genetics Base Sequence Genetic Variation Humans Molecular Sequence Data Nucleic Acid Conformation Operon Phylogeny RNA, Ribosomal/genetics RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Species Specificity Whipple Disease/microbiology
Chemicals
RNA, Ribosomal RNA, Ribosomal, 16S RNA, Ribosomal, 23S RNA, Ribosomal, 5S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Maiwald M
Departments of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA.
von Herbay A
Lepp P W
Relman D A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.11.3292-3297.2000
Published
2000-06-00
Pages
3292-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94522
Subset
IM
Databases
GENBANK
AF190686, AF190687, AF190688, AF190689, AF190690, AF190691
External Links
PubMed source DOI: 10.1128/JB.182.11.3292-3297.2000 PMC Full Text View journal details
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