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PMID: 1079511 Published · ppublish English Journal Article

Limiting dilution analysis of helper T-cell function.

Immunology ·Vol. 28 ·No. 6 ·1975-06-00 ·Pages 1135-48

Waldmann H, Lefkovits I, Quintáns J

Abstract

Limiting dilution analysis has been applied to the study of T-cell 'helper' function in vitro. Using the microculture system one can estimate the numbers of (a) 'helper' T cells involved in specific collaboration with B cells and (b) those T cells which are able, on being activated by their specific antigen, to facilitate the response of B cells to another antigen. Such studies have enabled us to demonstrate that: (1) a single 'helper' T cell was able to activate a single B-cell precursor to detectable antibody production; (2) the 'helper' function of primed T cells was radio-resistant; (3) a minimal estimate of 'helper' frequencies could be obtained in defined cell populations; (4) nonspecific facilitation was directed towards virtually all available B cells of a given specificity if these were challenged with their appropriate particulate antigen; (5) the microculture system offers the opportunity to determine whether specific and non-specific T-cell 'helper' effects are a consequence of the activity of one T-cell type or of differenct subpopulations of T cells.

MeSH Terms
Animals Antibody Specificity Antibody-Producing Cells/radiation effects B-Lymphocytes/immunology Carrier Proteins Cell Count Erythrocytes/immunology Haptens Hemocyanins/immunology Immunologic Memory Mice Mice, Inbred CBA Mice, Nude Perissodactyla/immunology Sheep/immunology T-Lymphocytes/immunology
Chemicals
Carrier Proteins Haptens Hemocyanins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Waldmann H
Lefkovits I
Quintáns J
References (28)
28 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1975-06-00
Pages
1135-48
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1445909
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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